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学者姓名：刘树滔
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Abstract ：
透明质酸（HA）是一种非硫酸化糖胺聚糖，2021年获批为新食品原料后，引起食品科研人员与产业界的广泛兴趣。近年来，越来越多的研究显示，HA的生物活性根据其分子质量的不同，发挥促炎或抗炎、促细胞增殖或抗增殖作用。为了高效、精准地提高HA在食品中的应用价值，有必要根据分子质量的差异性，全面归纳总结HA在食品相关领域的研究进展。本文概述HA的应用开发进展，重点分析不同分子质量HA的制备方法、细胞功能、消化吸收特性，从维持稳态、缓解炎症两个维度分析HA对肠道的调节作用，以期促进HA在营养与健康产业的精准开发及应用。
Keyword ：
分子质量 分子质量 制备 制备 生物学功能 生物学功能 肠道 肠道 透明质酸 透明质酸
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| GB/T 7714 | 滕薇 , 刘树滔 , 吴金鸿 et al. 透明质酸的制备、功能特性及其调节肠道健康的研究进展 [J]. | 中国食品学报 , 2024 , 24 (07) : 401-413 . |
| MLA | 滕薇 et al. "透明质酸的制备、功能特性及其调节肠道健康的研究进展" . | 中国食品学报 24 . 07 (2024) : 401-413 . |
| APA | 滕薇 , 刘树滔 , 吴金鸿 , 张勇 . 透明质酸的制备、功能特性及其调节肠道健康的研究进展 . | 中国食品学报 , 2024 , 24 (07) , 401-413 . |
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With an increasing incidence rate,ulcerative colitis (UC) is an idiopathic inflammation of the colon causing diarrhea and blood in the stool. Existing studies have shown that oxidative stress is an important factor leading to UC, while superoxide dismutase (SOD) has a certain effect on relieving UC symptoms. To improve its bioavailability, superoxide dismutase (SOD) was self-assembled into nanoparticles (ΔSOD) by heating it. The activity, transmembrane capacity and radical scavenging capacity of ΔSOD were significantly better than those of native SOD. ΔSOD liposomes (L-ΔSOD) were prepared by reverse-phase evaporation and orally administered to mice with UC. The results showed that compared with the positive control and model groups, L-ΔSOD significantly ameliorated UC, reduced disease activity index (DAI) score, restored the morphology of colon tissue, increased the length of colon, reduced the density of colon and spleen coefficient, and restored the integrity of mucosal epithelium. Besides, L-ΔSOD could reduce the contents of malondialdehyde (MDA) and pro-inflammatory factors in colon tissue and increase the levels of glutathione peroxidase (GSH-Px) and tight junction protein mRNA expression. These results confirmed that oral L-ΔSOD has a good ameliorative effect on UC, which will lay the foundation for the application of SOD in promoting human health. © 2023, China Food Publishing Company. All right reserved.
Keyword ：
Antioxidants Antioxidants Biochemistry Biochemistry Disease control Disease control Diseases Diseases Liposomes Liposomes Mammals Mammals Nanoparticles Nanoparticles Restoration Restoration Superoxide dismutase Superoxide dismutase Tissue Tissue
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| GB/T 7714 | Li, Qiaoling , Li, Henian , Guo, Jingke et al. Ameliorative Effect of Orally Administered Nano-Superoxide Dismutase Liposomes on Ulcerative Colitis in Mice [J]. | Food Science , 2023 , 44 (1) : 166-174 . |
| MLA | Li, Qiaoling et al. "Ameliorative Effect of Orally Administered Nano-Superoxide Dismutase Liposomes on Ulcerative Colitis in Mice" . | Food Science 44 . 1 (2023) : 166-174 . |
| APA | Li, Qiaoling , Li, Henian , Guo, Jingke , Zhu, Zeliang , Teng, Wei , Hu, Yujia et al. Ameliorative Effect of Orally Administered Nano-Superoxide Dismutase Liposomes on Ulcerative Colitis in Mice . | Food Science , 2023 , 44 (1) , 166-174 . |
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Instant rice with added coarse grain (IR/CG) is a new type of coarse grain product which is self-heating and convenient to eat and has the nutritional value of coarse grains. However, its health effects are not clear yet. Therefore, in this study, the effect of IR/CG on glucose and lipid metabolism was explored in mice with type 2 diabetes mellitus (T2DM) induced by high fat diet (HFD) combined with intraperitoneal injection of streptozotocin. The mice were fed on HFD alone (model), its mixture with IR/CG in different ratios: 25% (low-dose IR/CG, IR/CG-L), 50% (medium-dose IR/CG, IR/CG-M), and 75% (high-dose IR/CG, IR/CG-H), IR/CG alone, or a basal diet (control). During the dietary intervention, the body mass, fasting blood glucose, food intake and glucose tolerance of the mice were measured. After the IR/CG intervention for four weeks, the organ coefficients were calculated, and the pathological changes in liver and pancreas tissues were examined by hematoxylin and eosin (HE) staining. Fasting insulin levels, blood glucose and lipid levels, and proinflammatory cytokines levels were determined. The experimental results showed that dietary supplementation of IR/CG improved blood glucose and lipid levels in T2DM mice to different extents, and this effect was more prominent in the IR/CG-H and IR/CG groups. In addition, IR/CG-H and IR/CG significantly mitigated insulin resistance, decreased insulin secretion, glucagon and HbA1c levels, and reduced oxidative stress injury and inflammatory cytokine levels in T2DM mice. By analyzing the organ index and pathological sections of liver and pancreas tissues, it was found that IR/CG intervention could effectively alleviate liver steatosis and partially restore the structure of the pancreatic islets in mice. Therefore, the substitution of high-fat diet by IR/CG has a significant effect on regulating blood glucose and lipids in T2DM mice, which can provide a theoretical basis for the development of self-heating rice products and functional foods with coarse-grain. © 2023 Chinese Chamber of Commerce. All rights reserved.
Keyword ：
blood glucose blood glucose instant rice with added coarse grain instant rice with added coarse grain type 2 diabetes mellitus type 2 diabetes mellitus
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| GB/T 7714 | Zhang, C. , Guo, J. , Hu, Y. et al. Effect of the Substitution of High-Fat Diet by Instant Rice with Added Coarse Grain on Glucose and Lipid Metabolism in Mice with Type 2 Diabetes Mellitus; [粗粮速食米替代高脂饮食对2型糖尿病小鼠糖脂代谢的影响] [J]. | Food Science , 2023 , 44 (9) : 96-103 . |
| MLA | Zhang, C. et al. "Effect of the Substitution of High-Fat Diet by Instant Rice with Added Coarse Grain on Glucose and Lipid Metabolism in Mice with Type 2 Diabetes Mellitus; [粗粮速食米替代高脂饮食对2型糖尿病小鼠糖脂代谢的影响]" . | Food Science 44 . 9 (2023) : 96-103 . |
| APA | Zhang, C. , Guo, J. , Hu, Y. , Wang, X. , Liu, S. . Effect of the Substitution of High-Fat Diet by Instant Rice with Added Coarse Grain on Glucose and Lipid Metabolism in Mice with Type 2 Diabetes Mellitus; [粗粮速食米替代高脂饮食对2型糖尿病小鼠糖脂代谢的影响] . | Food Science , 2023 , 44 (9) , 96-103 . |
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Ulcerative colitis (UC) is an idiopathic intestinal disease with an increasing incidence, and all the therapeutic drugs have different degrees of side effects. Studies have found that collagen peptide can relieve the symptoms of UC, and is safe and healthy. However, the current research is mainly limited to the prevention effect of UC, and there is still a lack of research on the therapeutic effect of UC. Eel skin is rich in collagen, but eel head is generally discarded in processing, resulting in a waste of resources. Meanwhile, there is a lack of research on eel skin collogen hydrolysates. In this study, collagen was extracted from eel skin and hydrolyzed in the hope of obtaining eel skin collagen hydrolysate (CH) with therapeutic effect on UC, so as to improve the utilization value of eel processing by-products. It was found that eel skin contained abundant collagen, accounting for 27.87% of the wet weight of the skin. The extracted collagen was of high purity and mainly type I collagen, and eel skin could be used as a high - quality source of collagen. Collagen was enzymatically hydrolyzed to obtain eel skin collagen hydrolysate (CH) with antioxidant activity, and CH was administered to DSS induced UC mouse model. The results showed that CH could safely and healthily reduce DAI score to 0 and improve colon status in UC Model mice compared with Positive control group and Model group. Pathological sections of colon tissue showed that CH could restore the integrity of mucosal epithelium and reduce inflammatory cell infiltration. Oxidative stress index of colon tissue showed that CH could reduce MDA content (0.75 nmol/mg protein) and MPO activity (0.11 U/g tissue) in colon tissue, and improve the antioxidant capacity of colon tissue and reduce oxidative stress level by increasing GSH content (22.92 umol/g pro) and activities of SOD (693.61 U/g pro), CAT (1.69 U/g pro) and GPx (141.29 U/g pro) antioxidant enzymes, so as to treat UC. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
Keyword ：
antioxidant antioxidant collagen collagen eel skin eel skin ulcerative colitis ulcerative colitis
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| GB/T 7714 | Li, H. , Hu, Y. , Zhang, C. et al. The Effect of Collagen Hydrolysate of Eel Skin on the Improvement of Colitis; [鳗鱼皮胶原蛋白水解物对结肠炎的改善作用] [J]. | Journal of Chinese Institute of Food Science and Technology , 2023 , 23 (4) : 96-105 . |
| MLA | Li, H. et al. "The Effect of Collagen Hydrolysate of Eel Skin on the Improvement of Colitis; [鳗鱼皮胶原蛋白水解物对结肠炎的改善作用]" . | Journal of Chinese Institute of Food Science and Technology 23 . 4 (2023) : 96-105 . |
| APA | Li, H. , Hu, Y. , Zhang, C. , Lin, J. , Guo, J. , Liu, S. . The Effect of Collagen Hydrolysate of Eel Skin on the Improvement of Colitis; [鳗鱼皮胶原蛋白水解物对结肠炎的改善作用] . | Journal of Chinese Institute of Food Science and Technology , 2023 , 23 (4) , 96-105 . |
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Oxidative stress is generally considered inseparable from the development and exacerbation of ulcerative colitis (UC). Therefore, reducing oxidative stress has become a possible way to alleviate UC. In this study, the therapeutic effects of different doses of liposome-embedded superoxide dismutase (L-SOD) on mice with DSS-induced UC were systematically investigated. The results showed that L-SOD significantly attenuated the signs of colitis in mice, including colonic shortening, diarrhoea, bloody stools, and histopathological changes. L-SOD ameliorated DSS-induced oxidative damage, increased SOD, catalase (CAT), and glutathione (GSH) activities, and decreased malondialdehyde (MDA) levels. In addition, L-SOD ameliorated the inflammatory response by inhibiting the expression of myeloperoxidase (MPO) and pro-inflammatory cytokines and protected barrier function by promoting the expression of the tight junction proteins occludin and ZO-1 in the colon. Importantly, the results demonstrated a bell-shaped distribution of therapeutic effects relative to the administered dose, with an optimal dose of 150 000 U kg(-1). These results indicate that L-SOD has great potential as an ingredient in functional foods for the prevention and mitigation of UC.
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| GB/T 7714 | Zhang, Chi , Hu, Yujia , Yuan, Yi et al. Liposome-embedded SOD attenuated DSS-induced ulcerative colitis in mice by ameliorating oxidative stress and intestinal barrier dysfunction [J]. | FOOD & FUNCTION , 2023 , 14 (9) : 4392-4405 . |
| MLA | Zhang, Chi et al. "Liposome-embedded SOD attenuated DSS-induced ulcerative colitis in mice by ameliorating oxidative stress and intestinal barrier dysfunction" . | FOOD & FUNCTION 14 . 9 (2023) : 4392-4405 . |
| APA | Zhang, Chi , Hu, Yujia , Yuan, Yi , Guo, Jingke , Li, Henian , Li, Qiaoling et al. Liposome-embedded SOD attenuated DSS-induced ulcerative colitis in mice by ameliorating oxidative stress and intestinal barrier dysfunction . | FOOD & FUNCTION , 2023 , 14 (9) , 4392-4405 . |
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The chitosan-coated alginate gel particles (CS/SA) and chitosan-coated alginate-shellac gel particles (CS/SA/Lac) were constructed to serve as a potential oral delivery system to enhance the stability and activity of superoxide dismutase (SOD) in this study. The encapsulation efficiency (EE), enzyme activity retention ratio (ER), particle structure, and SOD release behavior, as well as the effect of shellac, were evaluated. The best formula is determined by the percentage of total effective activity (PTEA).The results showed that CS/SA prepared by 0.30 parts per thousand chitosan, 1.00% CaCl2, 2.00% sodium alginate (M/G = 1:1, Mw: 38,857 kDa), and gelation solution pH 5.50 had the best chitosan shell, the strongest gel structure and the maximum PTEA which was 10.75 +/- 1.45%. Based on the optimal formula, addition of shellac that formed shellac-Ca2+ microspheres in the gel network was averse to SOD encapsulation, but it significantly improved the stability of SOD in gastric conditions. By adding 15.00% shellac, the PTEA reached 16.30 +/- 1.64%. At optimal formulation, SOD encapsulated in CS/SA was completely released in the stimulated intestinal fluid and accumulative release enzyme activity (EA) was 966.62 +/- 103.75 (U) after 6 h. However, the CS/SA/Lac with a low swelling ratio only released SOD in stimulated colonic fluid and the EA value reached 1692.28 +/- 101.82 (U) after 1 h. These findings demonstrated that CS/SA and CS/SA/Lac were excellent candidates for SOD oral administration, which was useful in the food and biomedical industries for developing functional products containing SOD.
Keyword ：
Chitosan-coated alginate Chitosan-coated alginate Chitosan-coated alginate gel particles Chitosan-coated alginate gel particles Intestinal delivery Intestinal delivery shellac gel particles shellac gel particles Superoxide dismutase Superoxide dismutase
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| GB/T 7714 | Zhu, Zeliang , Yuan, Yi , Guo, Jingke et al. Encapsulation of SOD in chitosan-coated gel particles of alginate or mixture of alginate and shellac for targeted intestinal delivery [J]. | FOOD HYDROCOLLOIDS , 2023 , 142 . |
| MLA | Zhu, Zeliang et al. "Encapsulation of SOD in chitosan-coated gel particles of alginate or mixture of alginate and shellac for targeted intestinal delivery" . | FOOD HYDROCOLLOIDS 142 (2023) . |
| APA | Zhu, Zeliang , Yuan, Yi , Guo, Jingke , Luo, Xiaolin , Liu, Shutao , Miao, Song . Encapsulation of SOD in chitosan-coated gel particles of alginate or mixture of alginate and shellac for targeted intestinal delivery . | FOOD HYDROCOLLOIDS , 2023 , 142 . |
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香辛料可以改善食品品质、色泽、风味和滋味，同时有多种保健功能，可用于散寒、除湿、疏通经络等。本研究目的为以性味甘、温的甘草、桂皮和生姜为原材料，研制一款可口且能作用于经络的天然香辛料饮料。饮料配方通过6～8人组成的感官小组进行感官评价来确定。对经络的作用通过观察受试者经络电压的变化来确定。结果表明，当甘草0 mg/mL、桂皮1.5 mg/mL、生姜0.5 mg/mL时，该香辛料组合饮料感官评价效果最佳。经络电压测量表明，7种香辛料组合（甘草、桂皮、生姜、甘草-桂皮、甘草-生姜、生姜-桂皮、甘草-生姜-桂皮）中生姜-桂皮组对人体经络电压作用最为突出，能够大幅度提高肝经、脾经和肾经的经络电压，显示对肝经、脾经和肾经具有特殊的作用。
Keyword ：
归经 归经 经络电压 经络电压 香辛料 香辛料
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| GB/T 7714 | 罗圆圆 , 郭静科 , 刘树滔 et al. 香辛料饮料研制及其对人体经络电压的作用 [J]. | 中国食品学报 , 2023 , 23 (09) : 109-119 . |
| MLA | 罗圆圆 et al. "香辛料饮料研制及其对人体经络电压的作用" . | 中国食品学报 23 . 09 (2023) : 109-119 . |
| APA | 罗圆圆 , 郭静科 , 刘树滔 , 李鹤年 , 黎俏灵 , 王梦田 . 香辛料饮料研制及其对人体经络电压的作用 . | 中国食品学报 , 2023 , 23 (09) , 109-119 . |
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粗粮速食米（instant rice with added coarse grain,IR/CG）是一种新型的粗粮制品，既具有自热米饭食用方便的特点，又具有粗粮谷物的营养价值，但其产生的健康影响并不明确，因此本研究通过高脂饮食（high fat diet,HFD）联合链脲佐菌素腹腔注射诱导小鼠产生糖尿病，探究IR/CG对2型糖尿病（type 2 diabetes mellitus,T2DM）小鼠糖脂代谢的作用。通过IR/CG与高脂饲料不同比例混合制备小鼠饲料，低、中、高剂量（25%（质量分数，下同）、50%、75%IR/CG）饲料以及100%IR/CG饲料分别记作IR/CG-L、IR/CG-M、IR/CG-H及IR/CG，利用上述饲料喂养小鼠，并与基础饲料、高脂饲料喂养的小鼠（分别记作Control、Model组）进行生理、生化指标等的比较。在饮食干预期间测定小鼠体质量、空腹血糖浓度、摄食量及葡萄糖耐量；IR/CG饮食干预4周后，计算小鼠脏器系数，对小鼠肝脏、胰腺进行苏木精-伊红染色，测定小鼠空腹胰岛素水平、血糖血脂相关指标，并测定小鼠促炎细胞因子水平。结果显示，IR/CG的摄入能够不同程度改善T2DM小鼠高血糖血脂水平，其中IR/CG-H、IR/CG组改善效果较为显著。此外，IR/CG-H、IR/CG能够显著改善小鼠胰岛素抵抗现象，减少胰岛素分泌、降低胰高血糖素及糖基化血红蛋白水平，减轻小鼠炎症状态。通过对肝脏、胰腺的脏器指数及病理学切片分析发现，IR/CG干预能够有效减轻小鼠肝脂肪变性，部分恢复胰岛结构。上述结果表明IR/CG替代HFD对T2DM小鼠具有显著的调节血糖血脂作用，本研究能为粗粮自热米饭产品及粗粮功能性食品的开发提供理论依据。
Keyword ：
2型糖尿病 2型糖尿病 粗粮速食米 粗粮速食米 血糖 血糖
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| GB/T 7714 | 张弛 , 郭静科 , 胡雨嘉 et al. 粗粮速食米替代高脂饮食对2型糖尿病小鼠糖脂代谢的影响 [J]. | 食品科学 , 2023 , 44 (09) : 96-103 . |
| MLA | 张弛 et al. "粗粮速食米替代高脂饮食对2型糖尿病小鼠糖脂代谢的影响" . | 食品科学 44 . 09 (2023) : 96-103 . |
| APA | 张弛 , 郭静科 , 胡雨嘉 , 王旭峰 , 刘树滔 . 粗粮速食米替代高脂饮食对2型糖尿病小鼠糖脂代谢的影响 . | 食品科学 , 2023 , 44 (09) , 96-103 . |
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溃疡性结肠炎（UC）是一种特发性肠道疾病，发病率不断上升，且治疗药物都存在不同程度的副作用。研究发现胶原蛋白肽可缓解UC症状，并且安全、健康。然而，目前研究主要局限于UC的预防作用，缺少对其治疗作用的研究。鳗鱼皮富含胶原蛋白，而鳗鱼头一般在加工中被丢弃，造成资源浪费，目前缺少对鳗鱼皮胶原蛋白水解物的研究。本研究尝试利用鳗鱼头中的鱼皮提取胶原蛋白并进行水解，希望得到对UC具有治疗作用的鳗鱼皮胶原蛋白水解物（CH），提高鳗鱼加工副产物的利用价值。研究发现鳗鱼皮所含胶原蛋白丰富，占鱼皮湿重的27.87%，纯度较高且主要为Ⅰ型胶原蛋白，可作为胶原蛋白的优质来源。对胶原蛋白进行酶解，得到具有抗氧化活性的CH，并给药UC模型小鼠，结果显示：与阳性对照和模型组相比，CH可以安全健康地降低UC模型小鼠的DAI评分至0，并改善结肠状态；结肠组织病理切片表明CH可以恢复黏膜上皮完整性，减轻炎性细胞浸润；结肠组织氧化应激指标表明CH可以降低结肠组织MDA含量（0.75 nmol/mg pro）和MPO活性（0.11 U/g tissue），并通过提高GSH含量（22.92 umol/g pro）和SOD(693.61 U/g pro)、CAT(1.69 U/g pro)、GPx(141.29U/g pro)3种抗氧化酶活性来提高结肠组织抗氧化能力，降低氧化应激水平，从而治疗UC。
Keyword ：
抗氧化 抗氧化 溃疡性结肠炎 溃疡性结肠炎 胶原蛋白 胶原蛋白 鳗鱼皮 鳗鱼皮
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| GB/T 7714 | 李鹤年 , 胡雨嘉 , 张弛 et al. 鳗鱼皮胶原蛋白水解物对结肠炎的改善作用 [J]. | 中国食品学报 , 2023 , 23 (04) : 96-105 . |
| MLA | 李鹤年 et al. "鳗鱼皮胶原蛋白水解物对结肠炎的改善作用" . | 中国食品学报 23 . 04 (2023) : 96-105 . |
| APA | 李鹤年 , 胡雨嘉 , 张弛 , 林婕 , 郭静科 , 刘树滔 . 鳗鱼皮胶原蛋白水解物对结肠炎的改善作用 . | 中国食品学报 , 2023 , 23 (04) , 96-105 . |
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目的:构建一种酶解—膜分离耦合的方法用来提高小分子透明质酸的生产效率。方法:利用酶生物反应罐与平板式聚醚砜超滤膜分离组件组成的连续式酶解—膜分离耦合反应体系。以膜通量、膜污染、透过液透明质酸质量浓度、产率为评价指标，系统地考察各因素对酶膜耦合体系内两种不同相对分子质量透明质酸分离效果的影响，并以产率为指标进一步通过正交试验优化酶膜耦合反应的工艺参数。结果:酶膜耦合反应体系制备小分子透明质酸的最优工艺参数为搅拌速度200 r/min、跨膜压力0.15 mPa、酶解时间4.0 h、加酶量5 g/100 g。该条件下可以快速制备并分离出两种不同相对分子质量(M_r)的小分子透明质酸，实现一步法同时制备M_r在1万～5万的低相对分子质量透明质酸(LMW-HA)和M_r<3 000的透明质酸寡糖(O-HA),且这两种不同相对分子质量透明质酸均具有清除DPPH自由基和羟自由基的能力。结论:酶解—膜分离耦合法可以连续同步生产具有不同相对分子质量和一定抗氧化性的LMW-HA和O-HA。
Keyword ：
工艺优化 工艺优化 抗氧化 抗氧化 连续制备 连续制备 透明质酸 透明质酸 酶膜耦合反应 酶膜耦合反应
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| GB/T 7714 | 滕薇 , 刘俊辉 , 吴金鸿 et al. 酶解—膜分离耦合连续制备抗氧化性小分子透明质酸 [J]. | 食品与机械 , 2023 , 39 (12) : 162-170 . |
| MLA | 滕薇 et al. "酶解—膜分离耦合连续制备抗氧化性小分子透明质酸" . | 食品与机械 39 . 12 (2023) : 162-170 . |
| APA | 滕薇 , 刘俊辉 , 吴金鸿 , 刘树滔 . 酶解—膜分离耦合连续制备抗氧化性小分子透明质酸 . | 食品与机械 , 2023 , 39 (12) , 162-170 . |
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