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学者姓名：孟春
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Abstract ：
Wuyi rock tea residue is rich in protein, making them a valuable protein source for producing angiotensin Iconverting enzyme (ACE) inhibitory peptides. In the present research, five novel ACE inhibitory peptides were isolated and identified from protein hydrolysate of Wuyi rock tea residue using Sephadex G-15, reversed phase high-performance liquid chromatography (RP-HPLC), and quadrupole time-of-flight mass spectrometry. The peptide sequences were identified as FPFPRPP, PPPRGP, PFPRPPH, LGHPW and LKFPDF, with IC50 values ranged from 0.271 mg/mL to 1.018 mg/mL, with FPFPRPP demonstrating the strongest activity. As indicated by the Lineweaver-Burk plot, FPFPRPP was a mixed-type inhibitor against ACE. Molecular docking studies further confirmed that FPFPRPP forms five hydrogen bonds with residues near the active sites of ACE, with a binding energy of -10.5 kcal/mol. Functional assay in human umbilical vein endothelial cells (HUVECs) showed that FPFPRPP significantly upregulated nitric oxide production and downregulated reactive oxygen species and endothelin-1 levels induced by angiotensin II, indicating its potential antihypertensive effect. These findings highlight Wuyi rock tea residue as a promising source of ACE inhibitory peptides and suggest FPFPRPP could be developed as a functional food ingredient with possible applications in hypertension management.
Keyword ：
Angiotensin I-Converting enzyme Angiotensin I-Converting enzyme Molecular mechanism Molecular mechanism Peptides Peptides Residue Residue Wuyi rock tea Wuyi rock tea
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| GB/T 7714 | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong et al. A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from Wuyi rock tea residue: Preparation, identification, and its potential molecular mechanism [J]. | LWT-FOOD SCIENCE AND TECHNOLOGY , 2025 , 216 . |
| MLA | Cao, Xiaoliang et al. "A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from Wuyi rock tea residue: Preparation, identification, and its potential molecular mechanism" . | LWT-FOOD SCIENCE AND TECHNOLOGY 216 (2025) . |
| APA | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong , Zhang, Zexiao , Guo, Songbin , Luo, Jinyan et al. A novel angiotensin I-converting enzyme (ACE) inhibitory peptide from Wuyi rock tea residue: Preparation, identification, and its potential molecular mechanism . | LWT-FOOD SCIENCE AND TECHNOLOGY , 2025 , 216 . |
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Curcumin's (Cur, C) utility is restricted by poor solubility, stability, and bioavailability. This study optimized zein (Z)-centered core-shell nanoparticles using soluble pea protein (Psup) or whey protein isolate (WPI, W) as the shell. pH-shifting enhanced Cur loading to 316.3 μg/mg (PsupZC) and 266.1 μg/mg (WZC), surpassing simple complexation, which left exposed Cur crystals after lyophilization. Core-shell ratios controls particle sizes (50–102 nm for PsupZC; 30–80 nm for WZC) with polydispersity index © 2024
Keyword ：
Core shell nanoparticles Core shell nanoparticles Glycoproteins Glycoproteins Hydrogen bonds Hydrogen bonds
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| GB/T 7714 | Huang, Wenting , Hong, Zhiping , Fu, Zhijie et al. Design of protein-based carriers for curcumin with tailored particle properties: Stability and mechanistic insights [J]. | Food Chemistry , 2025 , 487 . |
| MLA | Huang, Wenting et al. "Design of protein-based carriers for curcumin with tailored particle properties: Stability and mechanistic insights" . | Food Chemistry 487 (2025) . |
| APA | Huang, Wenting , Hong, Zhiping , Fu, Zhijie , Meng, Chun , Mao, Yuhong . Design of protein-based carriers for curcumin with tailored particle properties: Stability and mechanistic insights . | Food Chemistry , 2025 , 487 . |
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The white rot fungus Cerrena unicolor 87613 has been previously shown to be a promising resource in laccase production, an enzyme with significant biotechnological applications. Conventional methods face technical challenges in improving laccase activity. Attempts are still being made to develop novel approaches for further enhancing laccase activity. This study aimed to understand the regulation of laccase activity in C. unicolor 87613 for a better exploration of the novel approach. Transcriptomic and metabolomic analyses were performed to identify key genes and metabolites involved in extracellular laccase activity. The findings indicated a strong correlation between the glutathione metabolism pathway and laccase activity. Subsequently, experimental verifications were conducted by manipulating the pathway using chemical approaches. The additive reduced glutathione (GSH) dose-dependently repressed laccase activity, while the GSH inhibitors (APR-246) and reactive oxygen species (ROS) inducer (H2O2) enhanced laccase activity. Changes in GSH levels could determine the intracellular redox homeostasis in interaction with ROS and partially affect the expression level of laccase genes in C. unicolor 87613 in turn. In addition, GSH synthetase was found to mediate GSH abundance in a feedback loop. This study suggests that laccase activity is negatively influenced by GSH metabolism and provides a theoretical basis for a novel strategy to enhance laccase activity by reprogramming glutathione metabolism at a specific cultivation stage.
Keyword ：
Cerrena unicolor Cerrena unicolor glutathione metabolism glutathione metabolism laccase activity laccase activity metabolome metabolome transcriptome transcriptome
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| GB/T 7714 | Zhang, Long-Bin , Qiu, Ting-Ting , Qiu, Xiu-Gen et al. Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613 [J]. | MICROBIOLOGY SPECTRUM , 2024 , 12 (2) . |
| MLA | Zhang, Long-Bin et al. "Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613" . | MICROBIOLOGY SPECTRUM 12 . 2 (2024) . |
| APA | Zhang, Long-Bin , Qiu, Ting-Ting , Qiu, Xiu-Gen , Yang, Wu-Wei-Jie , Ye, Xiu-Yun , Meng, Chun . Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613 . | MICROBIOLOGY SPECTRUM , 2024 , 12 (2) . |
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Wuyi rock tea residues, generated after brewing or aroma extraction, are rich in nutrients. This study aimed to prepare ACE inhibitory peptides from the protein hydrolysate of Wuyi rock tea residues and investigate their stability. The optimal proteolysis conditions for neutral protease were optimized as: a substrate concentration of 2% (w/v), pH 7.0, hydrolysis temperature of 45 degrees C, enzymatic digestion time of 3 h, and enzyme dosage of 6800 U/g. Under these conditions, the ACE inhibition rate of protein hydrolysate of Wuyi rock tea residues reached 70.47 +/- 0.29% at a concentration of 0.5 mg/mL. Additionally, the prepared ACE inhibitory peptides demonstrated excellent stability against acid, base, heat, and certain metal ions. These findings suggest that the proteins extracted from Wuyi rock tea residues can serve as a promising source to produce highly active ACE inhibitory peptides. These peptides could be utilized in the development of nutraceuticals, functional foods, and pharmaceuticals.
Keyword ：
ACE inhibitory peptides ACE inhibitory peptides response surface methodology (RSM) response surface methodology (RSM) stability stability Wuyi rock tea residues Wuyi rock tea residues
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| GB/T 7714 | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong et al. Preparation and stability of angiotensin-I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of Wuyi rock tea residues [J]. | CYTA-JOURNAL OF FOOD , 2024 , 22 (1) . |
| MLA | Cao, Xiaoliang et al. "Preparation and stability of angiotensin-I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of Wuyi rock tea residues" . | CYTA-JOURNAL OF FOOD 22 . 1 (2024) . |
| APA | Cao, Xiaoliang , Zhou, Huiyuan , Xie, Jindong , Huang, Linxia , Guo, Songbin , Zhang, Zexiao et al. Preparation and stability of angiotensin-I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of Wuyi rock tea residues . | CYTA-JOURNAL OF FOOD , 2024 , 22 (1) . |
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目的 探究益景汤对早期糖尿病视网膜病变(DR)大鼠视网膜组织中微小RNA(miRNA)表达的影响,筛选出益景汤干预早期DR的靶点miRNA.方法 将20只2月龄雄性SD大鼠使用链脲佐菌素建立糖尿病大鼠模型,随机分为模型组(MG)和益景汤组(YJG),另将常规喂养的大鼠设为对照组(CG),每组10只.YJG组大鼠每日灌胃益景汤12.50 g/kg,CG、MG组大鼠灌胃等体积0.9%氯化钠注射液,均干预16周.给药完成后记录大鼠体质量,摘除双侧眼球剥离视网膜,提取总RNA,PCR扩增构建小RNA文库,采用高通量测序技术对该文库进行测序.对差异miRNA进行靶基因预测,并对靶基因进行基因本体论(GO)、Pathway分析,选择差异倍数较大的miRNA进行PCR验证.结果 (1)已知miRNA差异:筛选出644个已知miRNA,其中差异miRNA共59个.与CG组比较,MG组35个miRNA上调、6 个miRNA下调,YJG组20个miRNA上调、4个miRNA下调;与MG组比较,YJG组6个miRNA上调、13个miRNA下调,差异均有统计学意义(均P＜0.05);其中,1个上调miRNA,11个下调miRNA为益景汤干预DM大鼠后,3组共同差异性表达miRNA.(2)新发现miRNA差异:3组筛选出242个新发现的miRNA,其中差异miRNA共105个.与CG组比较,MG组50个miRNA上调,15个miRNA下调;与CG组比较,YJG组55个miRNA上调,20个miRNA下调;与MG组比较,YJG组16个miRNA上调,6个miRNA下调,差异均有统计学意义(均P＜0.05).(3)差异miRNA生物学功能和通路:差异基因主要涉及生物学过程方面的多种蛋白的转录调节;靶基因关联的通路主要是肿瘤相关的信号通路和糖脂代谢相关通路.(4)PCR验证:MG组大鼠视网膜miR-673-3p表达低于CG组,余11个miRNA表达均高于CG组;YJG组大鼠视网膜miR-673-3p、miR-23b-5p表达均高于MG组,余10个miRNA表达均低于MG组,差异均有统计学意义(均P＜0.05).除miR-23b-5p外,其余11个miRNA与上述RNA检测结果一致.结论 miR-673、miR-1、miR-133、miR-214、miR-23b、miR-31a、miR-451等可能是益景汤干预早期DR微血管损害的靶点miRNA.
Keyword ：
miRNA miRNA 益景汤 益景汤 糖尿病视网膜病变 糖尿病视网膜病变 靶点 靶点
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| GB/T 7714 | 王丽 , 邱心悦 , 孟春 et al. 益景汤对早期糖尿病视网膜病变大鼠视网膜miRNA表达的影响 [J]. | 中国中医眼科杂志 , 2024 , 34 (3) : 205-213 . |
| MLA | 王丽 et al. "益景汤对早期糖尿病视网膜病变大鼠视网膜miRNA表达的影响" . | 中国中医眼科杂志 34 . 3 (2024) : 205-213 . |
| APA | 王丽 , 邱心悦 , 孟春 , 刘光辉 . 益景汤对早期糖尿病视网膜病变大鼠视网膜miRNA表达的影响 . | 中国中医眼科杂志 , 2024 , 34 (3) , 205-213 . |
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OBJECTIVE: To investigate the effects of emodin on alkali burn -induced corneal inflammation and neovascularization. METHODS: The ability of emodin to target vascular endothelial growth factor receptor 2 (VEGFR2) was predicted by molecular docking. The effects of emodin on the invasion, migration, and proliferation of human umbilical vein endothelial cells (HUVEC) were determined by cell counting kit -8, Transwell, and tube formation assays. Analysis of apoptosis was performed by flow cytometry. CD31 levels were examined by immunofluorescence. The abundance and phosphorylation state of VEGFR2, protein kinase B (Akt), signal transducer and activator of transcription 3 (STAT3), and P38 were examined by immunoblot analysis. Corneal alkali burn was performed on 40 mice. Animals were divided randomly into two groups, and the alkali -burned eyes were then treated with drops of either 10 mu M emodin or phosphate buffered saline (PBS) four times a day. Slit - lamp microscopy was used to evaluate inflammation and corneal neovascularization (CNV) in all eyes on Days 0, 7, 10, and 14. The mice were killed humanely 14 d after the alkali burn, and their corneas were removed and preserved at -80 celcius until histological study or protein extraction. RESULTS: Molecular docking confirmed that emodin was able to target VEGFR2. The findings revealed that emodin decreased the invasion, migration, angiogenesis, and proliferation of HUVEC in a dose -dependent manner. In mice, emodin suppressed corneal inflammatory cell infiltration and inhibited the development of corneal neovascularization induced by alkali burn. Compared to those of the PBS -treated group, lower VEGFR2 expression and CD31 levels were found in the emodin- treated group. Emodin dramatically decreased the expression of VEGFR2, p-VEGFR2, p-Akt, p-STAT3, and p -P38 in VEGF-treated HUVEC. CONCLUSION: This study provides a new avenue for evaluating the molecular mechanisms underlying corneal inflammation and neovascularization. Emodin might be a promising new therapeutic option for corneal alkali burns.
Keyword ：
alkali burn alkali burn corneal inflammation corneal inflammation corneal neovascularisation corneal neovascularisation emodin emodin signal transduction signal transduction vascular endothelial growth factor receptor-2 vascular endothelial growth factor receptor-2
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| GB/T 7714 | Zheng, Xueying , Guo, Liang , Lai, Siyi et al. Emodin suppresses alkali burn-induced corneal inflammation and neovascularization by the vascular endothelial growth factor receptor 2 signaling pathway [J]. | JOURNAL OF TRADITIONAL CHINESE MEDICINE , 2024 , 44 (2) : 268-276 . |
| MLA | Zheng, Xueying et al. "Emodin suppresses alkali burn-induced corneal inflammation and neovascularization by the vascular endothelial growth factor receptor 2 signaling pathway" . | JOURNAL OF TRADITIONAL CHINESE MEDICINE 44 . 2 (2024) : 268-276 . |
| APA | Zheng, Xueying , Guo, Liang , Lai, Siyi , Li, Fengyue , Liang, Mingli , Liu, Wanting et al. Emodin suppresses alkali burn-induced corneal inflammation and neovascularization by the vascular endothelial growth factor receptor 2 signaling pathway . | JOURNAL OF TRADITIONAL CHINESE MEDICINE , 2024 , 44 (2) , 268-276 . |
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目的:观察可溶性糖蛋白130(sgp130)对糖尿病(DM)小鼠视网膜p-STAT3及VEGF-A表达的影响,探讨sgp130防治糖尿病视网膜病变(DR)炎性损害的可能性.方法:小鼠45只被随机分为正常组、DM组和sgp130组.采用链脲佐菌素对DM组和sgp130组进行DM造模.正常组、DM组不做特殊干预,sgp130组在第1、5wk被给予1.5mg/mL sgp1302μL进行玻璃体腔注射治疗.10wk后处死所有小鼠,检查视网膜组织IL-6、p-STAT3、VEGF-A等蛋白的表达.结果:在第10wk时,DM组视网膜IL-6、p-STAT3、VEGF-A表达较正常组升高(均P<0.01).sgp130组p-STAT3、VEGF-A表达水平较DM组低(均P<0.01).结论:sgp130可以选择性拮抗IL-6反式信号传导通路,下调下游炎性因子VEGF-A的表达,可以用于干预DM引起的IL-6相关性视网膜炎性损害.
Keyword ：
p-STAT3 p-STAT3 可溶性糖蛋白130(sgp130) 可溶性糖蛋白130(sgp130) 炎症 炎症 白介素6 白介素6 糖尿病视网膜病变 糖尿病视网膜病变 血管内皮生长因子A(VEGF-A) 血管内皮生长因子A(VEGF-A)
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| GB/T 7714 | 刘光辉 , 史常旋 , 洪雅军 et al. sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响 [J]. | 国际眼科杂志 , 2023 , 23 (3) : 375-378 . |
| MLA | 刘光辉 et al. "sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响" . | 国际眼科杂志 23 . 3 (2023) : 375-378 . |
| APA | 刘光辉 , 史常旋 , 洪雅军 , 郑永征 , 王航 , 孟春 . sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响 . | 国际眼科杂志 , 2023 , 23 (3) , 375-378 . |
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为研究外源酶对陈年武夷岩茶挥发性成分的影响,运用气相色谱-质谱联用技术(gas chromatography-mass spectrometry,GC-MS)、感官评价和香气活性值(odor activity value,OAV)方法对酶处理后的武夷岩茶进行分析.GC-MS分析结果表明,纤维素酶、果胶酶、漆酶和β-葡萄糖苷酶质量比为2:1:2:3时有利于香气物质的释放,尤其对醇类、酯类及酮类香气物质释放贡献较大.感官评价和OAV值的结果表明,酶的添加能明显提高武夷岩茶的花香、甜香和木香,复合酶的处理效果最佳,其次是β-葡萄糖苷酶.经复合酶处理后己醛、2-己烯醛和(E,E)-2,4-己二烯醛等物质含量降低,OAV减小.上述研究表明,纤维素酶、果胶酶、漆酶、β-葡萄糖苷酶及其复合酶的添加均可明显改善陈年武夷岩茶香气品质.
Keyword ：
β-葡萄糖苷酶 β-葡萄糖苷酶 果胶酶 果胶酶 武夷岩茶 武夷岩茶 气相色谱-质谱法 气相色谱-质谱法 漆酶 漆酶 纤维素酶 纤维素酶 香气活性值 香气活性值
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| GB/T 7714 | 谢李玲 , 薛婉茹 , 李丹阳 et al. 外源酶对陈年武夷岩茶香气品质的改善作用 [J]. | 食品研究与开发 , 2023 , 44 (5) : 155-164 . |
| MLA | 谢李玲 et al. "外源酶对陈年武夷岩茶香气品质的改善作用" . | 食品研究与开发 44 . 5 (2023) : 155-164 . |
| APA | 谢李玲 , 薛婉茹 , 李丹阳 , 周惠媛 , 孟春 , 洪晶 . 外源酶对陈年武夷岩茶香气品质的改善作用 . | 食品研究与开发 , 2023 , 44 (5) , 155-164 . |
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Because of the health benefits and economic opportunities, extracting bioactive peptides from plant proteins, often food processing by-products, garners significant interest. However, the high enzyme costs and the emergence of bitter peptides have posed significant challenges in production. This study achieved the immobilization of Alcalase and Flavorzyme using cost-effective SiO2 microparticles. Mussel-inspired chemistry and biocompatible polymers were employed, with genipin replacing glutaraldehyde for safer crosslinking. This approach yielded an enzyme loading capacity of approximately 25 mg/g support, with specific activity levels reaching around 180 U/mg for immobilized Alcalase (IA) and 35 U/mg for immobilized Flavorzyme (IF). These immobilized proteases exhibited improved activity and stability across a broader pH and temperature range. During the hydrolysis of soy proteins, the use of immobilized proteases avoided the thermal inactivation step, resulting in fewer peptide aggregates. Moreover, this study applied peptidomics and bioinformatics to profile peptides in each hydrolysate and identify bioactive ones. Cascade hydrolysis with IA and IF reduced the presence of bitter peptides by approximately 20%. Additionally, 50% of the identified peptides were predicted to have bioactive properties after in silico digestion simulation. This work offers a cost-effective way of generating bioactive peptides from soy proteins with reducing potential bitterness.
Keyword ：
Alcalase Alcalase bioactivity bioactivity bitterness bitterness Flavorzyme Flavorzyme immobilization immobilization peptidomics peptidomics soy proteins soy proteins
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| GB/T 7714 | Mao, Yuhong , Chen, Lan , Zhang, Luyan et al. Synergistic Hydrolysis of Soy Proteins Using Immobilized Proteases: Assessing Peptide Profiles [J]. | FOODS , 2023 , 12 (22) . |
| MLA | Mao, Yuhong et al. "Synergistic Hydrolysis of Soy Proteins Using Immobilized Proteases: Assessing Peptide Profiles" . | FOODS 12 . 22 (2023) . |
| APA | Mao, Yuhong , Chen, Lan , Zhang, Luyan , Bian, Yangyang , Meng, Chun . Synergistic Hydrolysis of Soy Proteins Using Immobilized Proteases: Assessing Peptide Profiles . | FOODS , 2023 , 12 (22) . |
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Alkali burn is a significant contributor to corneal injury. Alkali burn-induced corneal inflammation often causes vision loss due to corneal neovascularization. Daphnetin (DAP) has been studied for its anti-inflammatory and antiangiogenic properties with encouraging results. Driven by those encouraging results, we sought to explore the effects of DAP in treating alkali burn-induced corneal inflammation and neovascularization and its mechanism of action. We found that the angiogenesis processes of human umbilical vein endothelial cells (HUVECs) induced by vascular endothelial growth factor A (VEGF-A) were primarily attenuated by treatment with DAP, including proliferation, migration, and tube formation. Treatment of DAP significantly suppressed the VEGF-Ainduced protein expression of VEGF receptor2 (VEGFR2), as well as the activation of downstream signal transducer and activator of transcription 3 (STAT3), AKT, and extracellular signal-regulated kinase (ERK) signaling. In the mouse corneal alkali burn model, the inflammatory cell infiltrations and neovascularization in the cornea caused by alkali burn were inhibited by 10 mu M DAP eye drops. Alkali burn-induced corneal protein expression of VEGF-A, VEGFR2, phosphorylated (p-)STAT3, p-AKT, and p-ERK in corneal tissue were reduced mainly by DAP. Moreover, the upregulation of inflammatory caused by alkali burn in the pathological process was significantly neutralized by DAP. Mechanistically, the inflammatory response could be alleviated by DAP in the way of inhibiting the expression levels of TLR4, p-NF-kappa B, NLRP3, ASC, Cleaved-caspase-1 (p20), mature-IL-113 (p17), and N-GSDM. In conclusion, our findings confirmed that the corneal inflammation and neovascularization caused by alkali burn could be inhibited by DAP in vitro and in vivo, elucidating the underlying mechanisms of its protective effects. DAP may have tremendous therapeutic potential for the treatment of corneal alkali burn.
Keyword ：
Alkali-burn injury Alkali-burn injury Cornea Cornea Daphnetin Daphnetin Inflammation Inflammation Neovascularization Neovascularization
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| GB/T 7714 | Yang, Tianye , Wang, Xiaoli , Guo, Liang et al. Daphnetin inhibits corneal inflammation and neovascularization on a mouse model of corneal alkali burn [J]. | INTERNATIONAL IMMUNOPHARMACOLOGY , 2022 , 103 . |
| MLA | Yang, Tianye et al. "Daphnetin inhibits corneal inflammation and neovascularization on a mouse model of corneal alkali burn" . | INTERNATIONAL IMMUNOPHARMACOLOGY 103 (2022) . |
| APA | Yang, Tianye , Wang, Xiaoli , Guo, Liang , Zheng, Fang , Meng, Chun , Zheng, Yongzheng et al. Daphnetin inhibits corneal inflammation and neovascularization on a mouse model of corneal alkali burn . | INTERNATIONAL IMMUNOPHARMACOLOGY , 2022 , 103 . |
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