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学者姓名：王建
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Abstract ：
The persistence of T-2 toxin in food and feed matrices renders it a pervasive contaminant, impacting both human and animal health. Traditional detection methods suffer from cumbersome instrumentation and intricate procedures, rendering on-site detection of T-2 toxin unfeasible. Therefore, we have constructed a real-time detection method for T-2 toxin detection by employing a target-responsive DNA hydrogel in conjunction with potassium iodide starch test paper. This method integrates both colorimetric and distance-based signal outputs, offering a streamlined and effective approach for the on-site detection of T-2 toxin. The specific binding of the target to the aptamer in the DNA hydrogel results in the collapse of the hydrogels structure, which changes the viscosity of the system and released horseradish peroxidase in the hydrogel wrapped, and then produces blue-purple marks of different lengths on the starch iodide papers to achieve the specific detection of T-2 toxin. Under optimized conditions, the assay exhibits a detection range spanning from 10 ng/mL to 10 mg/mL for the toxin, with a detection limit of 12.83 ng/mL. The proposed method has been successfully applied for the detection of real corn samples with satisfied result. Such colorimetric-distance dual signal detection method offers notable advantages, including straightforward operation, clear signal interpretation, and practical utility. Its implementation enables rapid, on-the-spot detection of T-2 toxin, particularly beneficial in resource-limited regions and less developed countries.
Keyword ：
Point-of-care testing Point-of-care testing Starch iodide paper Starch iodide paper T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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| GB/T 7714 | Deng, Ye , Lin, Jiarong , Wang, Jingxuan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper [J]. | MICROCHEMICAL JOURNAL , 2025 , 210 . |
| MLA | Deng, Ye et al. "Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper" . | MICROCHEMICAL JOURNAL 210 (2025) . |
| APA | Deng, Ye , Lin, Jiarong , Wang, Jingxuan , Lin, Yue , Luo, Fang , Weng, Zuquan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper . | MICROCHEMICAL JOURNAL , 2025 , 210 . |
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Real time detection of hydrogen sulfide (H2S) in deep brain regions is of great significance. Photoelectrochemical (PEC) biosensors hold promise for real-time monitoring but low sensitivity due to the use of small electrode restrained its application. In this study, a miniaturized sensor that integrates organic photoelectrochemical transistors (OPECTs) with optical fiber (OF) microelectrodes was designed for the first time, which can amplify the small PEC signal into large channel current change. A conductive gold layer and cuprous oxide nanoparticles (Cu2O NPs) were sequentially deposited onto the OF, followed by a Nafion polymer coating to enhance anti-interference capabilities. Cu2O reacts with H2S to generate Cu2S. The resulting Cu2O/Cu2S heterojunction induces changes in the PEC signal and channel current. Compared to a conventional PEC system, the OPECT sensor has higher sensitivity and lower detection limit, enabling monitoring of H2S concentration fluctuations within the rat brain, which demonstrates the approach's capability for highly sensitive in vivo biomarker detection.
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| GB/T 7714 | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7467-7475 . |
| MLA | Zeng, Yulan et al. "Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7467-7475 . |
| APA | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui , Luo, Fang , Qiu, Bin , Fu, Fengfu et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7467-7475 . |
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Herein, we present an innovative electrochemiluminescence (ECL) biosensor for the ultrasensitive detection of N-nitrosodimethylamine (NDMA). The biosensor utilizes a triple signal amplification strategy, combining rolling circle amplification (RCA), CRISPR/Cas12a-driven hyperbranched rolling circle amplification (HRCA), and electrostatic repulsion with size exclusion effects from vertically ordered mesoporous silica film (VMSF)/indium tin oxide (ITO) on double-stranded DNA (dsDNA)-Ru(phen)3 2+ complexes. In this system, aptamers and circular DNA undergo RCA reactions, followed by the CRISPR/Cas12a-mediated HRCA process, producing abundant dsDNA. The electropositive ECL indicator, namely Ru(phen)3 2+, was subsequently adsorbed onto the electronegative dsDNA, forming dsDNA-Ru(phen)3 2+ complexes. These complexes are subjected to electrostatic repulsion and size exclusion by the VMSF-modified ITO electrode, resulting in a lower ECL intensity. Upon introducing NDMA, the aptamer preferentially binds to NDMA, thereby preventing the formation of long dsDNA. This process releases free Ru(phen)3 2+, which diffuses to the electrode surface through narrow mesoporous channels via electrostatic adsorption. Consequently, an enhanced and strong ECL signal is observed. The integration of VMSF enhances selectivity and sensitivity by excluding larger impurities and promoting the electrostatic repulsion of dsDNA-Ru(phen)3 2+ complexes near the electrode surface. Additionally, the CRISPR/Cas12a system eliminates the formation of primer dimers and reduces false positives through its unique cis- and trans-cleavage activities. The biosensor demonstrated excellent performance with a linear correlation between the ECL signal and NDMA concentration in the range spanning from 10 pg/mL to 10 mu g/mL, achieving a low limit of detection of 5.33 pg/mL. This platform offers a reliable and robust solution for detecting NDMA in complex matrices, making it a promising tool for environmental monitoring, public health, and safety applications.
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| GB/T 7714 | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (10) : 5828-5835 . |
| MLA | Zheng, Zhenjie et al. "Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification" . | ANALYTICAL CHEMISTRY 97 . 10 (2025) : 5828-5835 . |
| APA | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da , Weng, Zuquan , Wang, Jian , Lin, Cuiying et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification . | ANALYTICAL CHEMISTRY , 2025 , 97 (10) , 5828-5835 . |
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Combined the electrostatic interaction of the negatively charged gold nanorods (AuNRs) (as acceptor) and Ru(bpy)(3)(2+) (as donor), an electrochemiluminescence resonance energy transfer (ECL-RET) sensor was constructed and applied for the detection of organophosphorus pesticides (OPs). Negatively charged AuNRs were synthesized by modifying AuNRs with polystyrene sulfonate (PSS) firstly, which can bind to Ru(bpy)(3)(2+) through electrostatic interaction so that the luminophore was absorbed by the acceptor, the resonance energy transfer occurred and only low ECL signal had been detected. Thiocholine can be produced by the hydrolysis process of acetylthiocholine (ATCh) with the help of acetylcholinesterase (AChE), which can bond with PSS-modified AuNRs (PSS-AuNRs) through gold-sulfur interaction, this caused the releasing of the adsorbed Ru(bpy)(3)(2+) into the solution and resulting in the restoration of the ECL intensity. However, the activity of AChE was inhibited by OPs, and the recovery process of the ECL signal was thus suppressed as well. In this study, chlorpyrifos was chosen as model target, the results indicated that the correlation between the ECL intensity and the logarithm of chlorpyrifos concentration showed remarkable linearity across 1 ng/mL to 1 mg/mL, achieving a detection limit of 0.51 ng/mL. The proposed system has been utilized for detecting OPs in real samples with satisfied results.
Keyword ：
Acetylcholinesterase Acetylcholinesterase Electrochemiluminescence Electrochemiluminescence Gold nanorods Gold nanorods Organophosphorus pesticides Organophosphorus pesticides Resonance energy transfer Resonance energy transfer
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| GB/T 7714 | Li, Zixin , Lin, Zeyu , Chen, Lifen et al. Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+ [J]. | TALANTA , 2025 , 281 . |
| MLA | Li, Zixin et al. "Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+" . | TALANTA 281 (2025) . |
| APA | Li, Zixin , Lin, Zeyu , Chen, Lifen , Lin, Yue , Luo, Fang , Lin, Cuiying et al. Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+ . | TALANTA , 2025 , 281 . |
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Background: Bulk-type conductive metal-organic frameworks (c-MOFs) had been applied to modify the electrode and used in electrochemical sensing because of the high conductive properties. But which still suffer from low mass permeability, restricted active site exposure, and poor accessibility due to the coordination saturation at metal sites in the bulk-type c-MOF. Recent studies have demonstrated that transforming bulk-type MOFs into MOFs nanosheets (NSs) can maximize the exposure of active sites and mass transfer. However, c-MOF NSs have rarely been applied in electrochemical sensing. Results: This study presents NSs type c-MOF Cu3(benzenehexathiol) (CuBHT), synthesized using a simple sacrificial template method. CuBHT NSs were modified onto a glassy carbon electrode (GCE) to prepare CuBHT NSs/ GCE, which was then applied to sense the model target ascorbic acid (AA), the system exhibits high sensitivity of 1.521 mA mM-1 cm-2 and a wide linear range of 1-789 mu M, low detection limit of 0.46 mu M. The sensitivity is 1.90 times higher than that of bulk-type CuBHT nanoparticles (NPs) modified GCE, which can be attributed to the CuBHT NSs having more exposed Cu sites on their surfaces. CuBHT NSs/GCE was then used to monitor AA levels in human sweat during daily activities or exercise, and the results indicated high reliability compared to the vitamin C ASA kit method. Significance: The design of c-MOF CuBHT NSs/GCE lead to better performance in terms of sensitivity and low detection limit in AA sensing compared to bulk-type nanoparticles. The AA sensing mechanism based on CuBHT was investigated, and the sensing system was demonstrated by detecting AA in sweat. This work advances both the fundamental understanding and practical applications of c-MOF NSs in AA sensing.
Keyword ：
Ascorbic acid Ascorbic acid Conductive metal-organic frameworks Conductive metal-organic frameworks Electrochemistry Electrochemistry Mechanism Mechanism Nanosheets Nanosheets
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| GB/T 7714 | Yuan, Runhao , Zhong, Xiaolong , Sun, Weiming et al. Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode [J]. | ANALYTICA CHIMICA ACTA , 2025 , 1353 . |
| MLA | Yuan, Runhao et al. "Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode" . | ANALYTICA CHIMICA ACTA 1353 (2025) . |
| APA | Yuan, Runhao , Zhong, Xiaolong , Sun, Weiming , Wang, Jian , Huang, Chuanhui , Lin, Zhenyu et al. Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode . | ANALYTICA CHIMICA ACTA , 2025 , 1353 . |
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Benefiting from the well-matched energy levels between Ru(phen)32+ and GaN, and the ability of Ru(phen)32+ to intercalate into the grooves of double-stranded DNA (dsDNA), an innovative organic photoelectrochemical transistor (OPECT)-based biosensor has been developed for the detection of human papillomavirus 16 (HPV-16), which was chosen as a model target. Trigger DNA immobilized on the gold-coated GaN surface can initiate a hybridization chain reaction (HCR), generating long-stranded dsDNA, which can be used to load a large amount of Ru(phen)32+. The produced dsDNA can also serve as a π-π-stacked hole-transporting nanowire, enhancing the efficiency of the hole transport between GaN and Ru(phen)32+. Under light irradiation, efficient energy level matching between GaN and Ru(phen)32+ promotes the transport efficiency of photogenerated carriers, leading to a significant increase in the drain-source current (IDS). In the presence of HPV-16, the CRISPR/Cas12a system is activated and the immobilized trigger DNA is cleaved, thereby preventing the formation of dsDNA through HCR. As a result, a smaller amount of Ru(phen)32+ is immobilized on the electrode surface, leading to a reduction in the output signal. The signal change exhibits a linear relationship with the target concentration in the range of 10.00 fM to 10.00 nM, achieving a detection limit as low as 2.88 fM, which is much better than that of the corresponding photoelectrochemical (PEC) biosensor. The system demonstrates excellent sensitivity and specificity in clinical sample analysis. © 2025 American Chemical Society
Keyword ：
Biosensors Biosensors Chemical detection Chemical detection DNA DNA Energy efficiency Energy efficiency Hole mobility Hole mobility Photoelectrochemical cells Photoelectrochemical cells Surface reactions Surface reactions
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| GB/T 7714 | Tian, Xuran , Zhang, Zhiqin , Zeng, Yulan et al. Ru(phen)32+-Sensitized GaN-Gated Organic Photoelectrochemical Transistor for Sensitive and Selective Detection of Human Papillomavirus DNA [J]. | Analytical Chemistry , 2025 , 97 (30) : 16682-16689 . |
| MLA | Tian, Xuran et al. "Ru(phen)32+-Sensitized GaN-Gated Organic Photoelectrochemical Transistor for Sensitive and Selective Detection of Human Papillomavirus DNA" . | Analytical Chemistry 97 . 30 (2025) : 16682-16689 . |
| APA | Tian, Xuran , Zhang, Zhiqin , Zeng, Yulan , Chen, Zhonghui , Lin, Yue , Luo, Fang et al. Ru(phen)32+-Sensitized GaN-Gated Organic Photoelectrochemical Transistor for Sensitive and Selective Detection of Human Papillomavirus DNA . | Analytical Chemistry , 2025 , 97 (30) , 16682-16689 . |
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A novel photoelectrochemical sensor based on TiO2/NiPc/ZIF-67 composite was developed for ammonia (NH3) detection in water, showing exceptional sensitivity and selectivity. The sensor was fabricated by depositing TiO2, NiPc, and ZIF-67 onto an ITO electrode. Under optimized conditions, it achieved an ultra-low detection limit of 0.031 nM, surpassing previous reports. A strong linear correlation (R2 = 0.998) was observed between the relative value of the photocurrent and the logarithm of NH3 concentration over the range of 0.1 nM to 10 mu M. TiO2 ensured stable photocurrent generation, while ZIF-67, a metal-organic framework with polyhedral morphology, adjustable pore size, high surface area (758.6 m2/g BET), and microporous structure, selectively enriched NH3. In competitive adsorption experiments, NH3 exhibited a slower adsorption rate compared to gases like CO, CH4, N2, and O2, minimizing interference. NiPc, a p-type semiconductor with an 18 pi-electron conjugated structure, coordinated with NH3 to promote NH3 oxidation and accelerate the reaction rate. The synergistic effect of NiPc and ZIF-67 enhanced the sensor performance, as demonstrated by cyclic voltammetry, which showed that the electrode potential of the oxidation process shifts negatively when both materials were modified on a glassy carbon electrode upon NH3 addition. In real water samples, the sensor displayed high selectivity, with minimal response to common interfering ions such as Cu2+, Mg2+, and Ca2+, ensuring accurate NH3 detection in complex environments.
Keyword ：
Composite material Composite material NH (3) detection NH (3) detection Nickel phthalocyanine Nickel phthalocyanine Photoelectrochemical Sensor Photoelectrochemical Sensor TiO2 TiO2 Zeolite imidazolate framework-67 Zeolite imidazolate framework-67
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| GB/T 7714 | Wu, Wenru , Xiong, Nuoxuan , Lin, Yue et al. Nickel phthalocyanine-catalyzed ammonia detection on a TiO2 photoelectrochemical sensor with ZIF-67 enrichment for environmental water analysis [J]. | WATER RESEARCH , 2025 , 286 . |
| MLA | Wu, Wenru et al. "Nickel phthalocyanine-catalyzed ammonia detection on a TiO2 photoelectrochemical sensor with ZIF-67 enrichment for environmental water analysis" . | WATER RESEARCH 286 (2025) . |
| APA | Wu, Wenru , Xiong, Nuoxuan , Lin, Yue , Luo, Fang , Lin, Cuiying , Qiu, Bin et al. Nickel phthalocyanine-catalyzed ammonia detection on a TiO2 photoelectrochemical sensor with ZIF-67 enrichment for environmental water analysis . | WATER RESEARCH , 2025 , 286 . |
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In this study, we present the innovative design and comprehensive evaluation of a novel point-of-care testing (POCT) methodology for the rapid and accurate detection of T-2 toxin, a potent mycotoxin with significant implications for food safety and human health. The cornerstone of this approach lies in the integration of a copper-based conductive metal-organic framework (Cu3(HHTP)2) with a target-responsive DNA hydrogel system, creating a dual-signal readout mechanism that significantly enhances detection sensitivity and specificity. Specifically, the hydrogel covers the material's surface metal active sites, which become exposed upon target addition due to hydrogel collapse. The released Cu3(HHTP)2 can catalyze the oxidation of 3,3 ',5,5 '-tetrame-thylbenzidine, resulting in a colorimetric and temperature change in the solution. This dual-mode detection strategy enables both qualitative assessment through direct visual inspection of color change and quantitative analysis by monitoring the solution temperature variation post laser irradiation with a thermometer. Under the optimized conditions, the detection system demonstrates a wide range spanning from 5 to 200 ng/mL, with a detection limit of 1.67 ng/mL. This method has been successfully demonstrated through the analysis of real-world samples, yielding encouraging results that underscore its reliability and effectiveness. The proposed dual-signal approach boasts advantages such as straightforward operational procedures, unambiguous signal outputs, and robust practicality, making it an attractive option for mycotoxin detection in resource-constrained settings and developing regions.
Keyword ：
Cu3(HHTP)2 Cu3(HHTP)2 Point-of-care testing Point-of-care testing T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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| GB/T 7714 | Lin, Jiarong , Deng, Ye , Lin, Yue et al. Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 438 . |
| MLA | Lin, Jiarong et al. "Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel" . | SENSORS AND ACTUATORS B-CHEMICAL 438 (2025) . |
| APA | Lin, Jiarong , Deng, Ye , Lin, Yue , Luo, Fang , Weng, Zuquan , Wang, Jian et al. Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel . | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 438 . |
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This study presents a smartphone-integrated portable platform for rapid lactic acid (LA) analysis using ratiometric fluorescent imaging. The system employs a dual-emission probe composed of glutathione-modified gold nanoclusters (GSH-AuNCs) and carbon quantum dots (CDs), enabling visual detection via fluorescence color transitions under 365 nm UV excitation. As LA concentration increases, the fluorescence shifts from blue to orange. Smartphone photography and RGB color analysis reveal a nonlinear sigmoidal correlation between the red-to-blue intensity ratio (R/B) and LA concentration, modeled with a coefficient of determination (R2 = 0.998). The method exhibits high selectivity and achieves recovery rates of 94.5-104.8 % in sweat samples, validated against LC-MS. This technology offers a novel, on-site solution for food quality assessment and health monitoring applications.
Keyword ：
Lactic acid Lactic acid Ratiometric fluorescence sensing Ratiometric fluorescence sensing Smartphone colorimetry Smartphone colorimetry
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| GB/T 7714 | Wu, Wen-Ru , Li, Jing , Lin, Yue et al. A portable platform integrated with smartphones for rapid lactic acid analysis via ratiometric fluorescent imaging [J]. | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 338 . |
| MLA | Wu, Wen-Ru et al. "A portable platform integrated with smartphones for rapid lactic acid analysis via ratiometric fluorescent imaging" . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 338 (2025) . |
| APA | Wu, Wen-Ru , Li, Jing , Lin, Yue , Luo, Fang , Lin, Cui-Ying , Qiu, Bin et al. A portable platform integrated with smartphones for rapid lactic acid analysis via ratiometric fluorescent imaging . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 338 . |
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Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface's charge density is then altered by the recognition of the target, leading to a change in the system's resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM-10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.
Keyword ：
aflatoxin B1 aflatoxin B1 microchannel microchannel portablesensor portablesensor resistance resistance surface charge density surface charge density
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| GB/T 7714 | Cai, Huabin , Huang, Yanling , Lin, Yue et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout [J]. | ACS SENSORS , 2024 , 9 (1) : 494-501 . |
| MLA | Cai, Huabin et al. "Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout" . | ACS SENSORS 9 . 1 (2024) : 494-501 . |
| APA | Cai, Huabin , Huang, Yanling , Lin, Yue , Luo, Fang , Chen, Lifen , Guo, Longhua et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout . | ACS SENSORS , 2024 , 9 (1) , 494-501 . |
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