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Abstract:
A ratiometric fluorescent aptasensor based on energy transfer between [Ru(bpy)(3)](2+) and silica quantum dots (silica QDs) for assaying OTA was fabricated. The aptamer for OTA was used as the gate to shield the fluorescent reagent [Ru(bpy)(3)](2+) into mesoporous silica nanoparticle (MSN). In the presence of OTA, the constrained [Ru(bpy)(3)](2+) was released from MSN due to a target-induced aptamer conformational change. The released [Ru(bpy)(3)](2+) adsorbed onto the negatively charged silica QDs through electrostatic interaction. This creates appearance of fluorescence from [Ru(bpy)(3)](2+) at 625 nm and decrease of the fluorescence from silica QDs at 442 nm owing to the energy transfer. The value of FL625nm/FL442nm was in proportion to the concentration of OTA in the range 0.5 similar to 100 ng mL(-1) with a LOD of 0.08 ng mL(-1). Practical applicability of this method was validated by the determination of OTA in flour samples. The sensing principle of this sensor
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MICROCHIMICA ACTA
ISSN: 0026-3672
Year: 2020
Issue: 5
Volume: 187
5 . 8 3 3
JCR@2020
5 . 4 0 0
JCR@2023
ESI Discipline: CHEMISTRY;
ESI HC Threshold:160
JCR Journal Grade:1
CAS Journal Grade:2
Cited Count:
WoS CC Cited Count: 14
SCOPUS Cited Count: 17
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 2
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