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author:

Zhu, Xi (Zhu, Xi.) [1] | Li, Wenjing (Li, Wenjing.) [2] | Lin, Liping (Lin, Liping.) [3] | Huang, Xiaojuan (Huang, Xiaojuan.) [4] | Xu, Huifeng (Xu, Huifeng.) [5] | Yang, Guidi (Yang, Guidi.) [6] | Lin, Zhenyu (Lin, Zhenyu.) [7] (Scholars:林振宇)

Indexed by:

Scopus SCIE

Abstract:

A ratiometric fluorescent aptasensor based on energy transfer between [Ru(bpy)(3)](2+) and silica quantum dots (silica QDs) for assaying OTA was fabricated. The aptamer for OTA was used as the gate to shield the fluorescent reagent [Ru(bpy)(3)](2+) into mesoporous silica nanoparticle (MSN). In the presence of OTA, the constrained [Ru(bpy)(3)](2+) was released from MSN due to a target-induced aptamer conformational change. The released [Ru(bpy)(3)](2+) adsorbed onto the negatively charged silica QDs through electrostatic interaction. This creates appearance of fluorescence from [Ru(bpy)(3)](2+) at 625 nm and decrease of the fluorescence from silica QDs at 442 nm owing to the energy transfer. The value of FL625nm/FL442nm was in proportion to the concentration of OTA in the range 0.5 similar to 100 ng mL(-1) with a LOD of 0.08 ng mL(-1). Practical applicability of this method was validated by the determination of OTA in flour samples. The sensing principle of this sensor

Keyword:

Aptamer Ochratoxin A Ratiometric fluorescent sensor [Ru(bpy)(3)](2+) Silica quantum dots

Community:

  • [ 1 ] [Zhu, Xi]Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou 350002, Fujian, Peoples R China
  • [ 2 ] [Li, Wenjing]Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou 350002, Fujian, Peoples R China
  • [ 3 ] [Lin, Liping]Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou 350002, Fujian, Peoples R China
  • [ 4 ] [Huang, Xiaojuan]Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou 350002, Fujian, Peoples R China
  • [ 5 ] [Yang, Guidi]Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou 350002, Fujian, Peoples R China
  • [ 6 ] [Zhu, Xi]Fujian Agr & Forestry Univ, Key Lab Crop Ecol & Mol Physiol, Fuzhou 35002, Fujian, Peoples R China
  • [ 7 ] [Li, Wenjing]Fujian Agr & Forestry Univ, Key Lab Crop Ecol & Mol Physiol, Fuzhou 35002, Fujian, Peoples R China
  • [ 8 ] [Yang, Guidi]Fujian Agr & Forestry Univ, Key Lab Crop Ecol & Mol Physiol, Fuzhou 35002, Fujian, Peoples R China
  • [ 9 ] [Xu, Huifeng]Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou, Fujian, Peoples R China
  • [ 10 ] [Xu, Huifeng]Fujian Univ Tradit Chinese Med, Fujian Key Lab Integrat Med Geriatr, Fuzhou, Fujian, Peoples R China
  • [ 11 ] [Lin, Zhenyu]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Minist Educ,Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China

Reprint 's Address:

  • [Yang, Guidi]Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou 350002, Fujian, Peoples R China;;[Yang, Guidi]Fujian Agr & Forestry Univ, Key Lab Crop Ecol & Mol Physiol, Fuzhou 35002, Fujian, Peoples R China;;[Xu, Huifeng]Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou, Fujian, Peoples R China;;[Xu, Huifeng]Fujian Univ Tradit Chinese Med, Fujian Key Lab Integrat Med Geriatr, Fuzhou, Fujian, Peoples R China

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Source :

MICROCHIMICA ACTA

ISSN: 0026-3672

Year: 2020

Issue: 5

Volume: 187

5 . 8 3 3

JCR@2020

5 . 4 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:160

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 14

SCOPUS Cited Count: 17

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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