A　functional　stainless　steel　microextraction　fiber　easily　prepared　by　in　situ　growing　metal-organic　framework　UiO-66　was　presented　and　used　for　high-performance　analysis　of　polycyclic　musks.　Via　the　robust　Ag-SH　bonding　reaction,　mercaptoacetic　acid　was　easily　anchored　on　Ag　film　to　provide　carboxyl　group　on　the　stainless　steel　fiber,　then　in　situ　grown　UiO-66　was　fulfilled　via　the　coordination　reaction　between　Zr4+　and　carboxyl　group.　Good　characteristics　including　large　surface　area,　high　thermal　stability,　and　good　adsorption　property　were　achieved.　Sensitive　detection　limits　(0.015-0.040　ng/L)　were　achieved　for　polycyclic　musks　by　coupling　with　gas　chromatography　with　mass　spectrometry,　and　it　could　be　stable　enough　for　150　extraction　cycles　without　a　significant　loss　of　extraction　efficiency.　Compared　with　the　classical　commercial　fibers,　2.2-11.4　times　higher　enhancement　factors　were　shown.　Applied　to　the　analysis　of　fortified　river　water　samples,　five　typical　polycyclic　musks　were　well　detected　with　the　recoveries　of　90.2-101.8%,　respectively.　It　showed　a　facile　approach　for　preparing　stainless　steel　microextraction　fiber　via　chemically　bonding　in　situ　grown　metal-organic　framework　for　high-performance　enrichment.