Most　of　the　current　analytical　methods　depend　largely　on　laboratory-based　analytical　techniques　that　require　expensive　and　bulky　equipment,　potentially　incur　costly　testing,　and　involve　lengthy　detection　processes.　With　increasing　requirements　for　point-of-care　testing　(POCT),　more　attention　has　been　paid　to　miniaturized　analytical　devices.　Miniaturized　electrochemical　(MEC)　sensors,　including　different　material-based　MEC　sensors　(such　as　DNA-,　paper-,　and　screen　electrode-based),　have　been　in　strong　demand　in　analytical　science　due　to　their　easy　operation,　portability,　high　sensitivity,　as　well　as　their　short　analysis　time.　They　have　been　applied　for　the　detection　of　trace　amounts　of　target　through　measuring　changes　in　electrochemical　signal,　such　as　current,　voltage,　potential,　or　impedance,　due　to　the　oxidation/reduction　of　chemical/biological　molecules　with　the　help　of　electrodes　and　electrochemical　units.　MEC　sensors　present　great　potential　for　the　detection　of　targets　including　small　organic　molecules,　metal　ions,　and　biomolecules.　In　recent　years,　MEC　sensors　have　been　broadly　applied　to　POCT　in　various　fields,　including　health　care,　food　safety,　and　environmental　monitoring,　owing　to　the　excellent　advantages　of　electrochemical　(EC)　technologies.　This　review　summarized　the　state-of-the-art　advancements　on　various　types　of　MEC　sensors　and　their　applications　in　POCT.　Furthermore,　the　future　perspectives,　opportunities,　and　challenges　in　this　field　are　also　discussed.　(C)　2019　Chinese　Chemical　Society　and　Institute　of　Materia　Medica,　Chinese　Academy　of　Medical　Sciences.　Published　by　Elsevier　B.V.　All　rights　reserved.