Monitoring　hypochlorite　anion　(ClO-)　in　living　cells　is　particularly　meaningful　and　valuable,　because　overexposure　of　the　ClO-　may　cause　a　potential　health　hazard　towards　animals　and　humans.　Considering　the　special　structure　and　properties　of　the　gemini　surfactant,　a　novel　amphiphilic　gemini-iridium　complex　Ir[(ppy-iso)(2)(bpy-tma(2)Br(2))]　(Ir-iso)　with　isoniazide　as　a　recognition　site　for　ClO-　was　designed.　The　Ir-iso　possessed　an　excellent　water-solubility　as　well　as　a　strong　ClO-　binding　capacity,　as　revealed　from　the　rapid　response　of　emission　signal　towards　ClO-.　It　was　worth　noting　that　such　probe　had　a　highly-specific　selectivity　with　a　low　detection　limit　(20.5　nM)　and　was　suitable　in　physiological　environment.　The　cell　viability　assay,　cell　imaging,　and　co-location　studies　further　proved　that　the　Ir-iso　had　little　cytotoxicity　and　was　specifically　localized　in　the　mitochondria　of　breast　cancer　cells,　being　a　promising　candidate　of　chemo-sensor　to　detect　the　endogenous　ClO-　in　living　cells.