An　innovative　bioetching　photoelectrochemical　(PEC)　mode　was　developed　by　using　enzyme-encapsulated　DNA　hydrogel　for　high-precision　quantification　of　glucose,　using　In2O3-CdS　nanocomposite　as　a　photoactive　matrix.　In　a　proof-of-concept　study,　horseradish　peroxidase　and　glucose　oxidase　were　encapsulated　in　situ　in　DNA　hydrogel　to　initiate　enzymatic　bioetching　of　CdS　nanoparticles　in　the　presence　of　target　glucose.　The　bioetching　reaction　of　CdS　inhibits　the　electron　transfer　efficiency　of　In2O3　and　CdS,　which　causes　a　change　in　photocurrent　intensity　and　thus　provides　a　powerful　bioetching　PEC　strategy　with　enzyme-encapsulated　DNA　hydrogel.