Amphibians　are　a　natural　source　of　abundant　antimicrobial　peptides　and　thus　have　been　widely　investigated　for　isolation　of　such　biomolecules.　Many　new　antimicrobial　peptide　families　have　been　discovered　from　amphibians.　In　this　study,　a　novel　antimicrobial　peptide　named　Limnonectes　fujianensis　Brevinvin　(LFB)　has　been　identified　in　the　skin　secretion　from　the　Fujian　large　headed　frog,　Limnonectes　fujianensis.　The　cDNA　sequence　was　cloned　from　a　skin　secretion　library　and　the　predicted　mature　peptide　was　identified　through　MS/MS　fragmentation　sequencing　of　reverse　phase　HPLC　fractions　on　the　same　sample.　LFB　was　predicted　to　be　an　amphipathic,　hydrophobic,　alpha　helical,　and　beta　turn　peptide　that　inserts　into　a　lipid　bilayer　in　order　to　kill　the　cells.　In　antimicrobial　assays,　a　synthetic　replicate　of　this　novel　antimicrobial　peptide　demonstrated　significant　activity　against　the　Gram-positive　bacterium　Staphylococcus　aureus,　the　Gram-negative　bacterium　Escherichia　coli　and　the　yeast,　Candida　albicans.　This　novel　peptide　was　highly　potent　(MIC　4.88　uM)　against　Gram-negative　bacterium,　and　also　has　the　ability　to　inhibit　the　growth　of　human　cancer　cell　lines　with　IC50　values　ranging　from　18.9　mu　M　down　to　2.0　mu　M.　These　findings　help　to　enrich　our　understanding　of　Brevinin-like　peptides.　Moreover,　the　data　presented　here　validate　frog　secretion　as　a　source　of　potential　novel　antimicrobial　peptides,　that　also　exhibit　anti-tumor　properties,　that　could　be　useful　for　the　treatment　of　cancer.