Osteoarthritis　(OA)　is　one　of　the　main　causes　of　disability　in　aging　population　and　the　effective　therapy　remains　elusive.　OA　involves　quantitative　and/or　qualitative　alterations　of　lubricin,　which　is　an　essential　mucinous　glycoprotein　secreted　by　synovial　fibroblasts　and　chondrocytes.　In　order　to　increase　the　expression　of　lubricin　in　cartilage　tissue　engineering,　we　explored　the　effect　of　kartogenin　(KGN),　transforming　growth　factor-beta　1　(TGF-beta　1)　and　morphogenetic　protein-7　(BMP-7)　on　the　expression　of　lubricin　in　bone-derived　mesenchymal　stem　cells　(BMSCs)　encapsulated　in　the　poly(ethylene　glycol)　diacrylate　(PEGDA)　hydrogels.　BMSCs　or　chondrocytes　were　incorporated　into　PEGDA　hydrogels　and　cultured　in　an　appropriate　microenvironment　fabricated　with　KGN,　TGF-beta　1,　and　BMP-7　to　detect　the　formed　substance　of　cartilage.　The　results　showed　that　BMSCs　and　chondrocytes　could　normally　proliferate　in　the　PEGDA　hydrogels.　The　BMSCs-PEGDA　constructs　were　treated　with　TGF-beta　1,　BMP-7,　and　KGN　and　achieved　higher　lubricin　expression　and　more　extracellular　matrices　production,　such　as　characteristic　glycosaminoglycans　(GAGs),　compared　with　other　groups.　These　results　suggested　that　combination　of　TGF-beta　1,　BMP-7,　and　KGN　could　enhance　the　level　of　lubricin　significantly　in　BMSCs-based　therapies,　which　may　be　a　new　method　for　the　treatment　of　joint　diseases.　This　study　provides　a　research　model　and　theoretical　basis　for　the　future　cartilage　regeneration　medicine　and　related　clinical　trials.