Amino　functionalized　surfaces　were　selectively　modified　via　the　combination　of　a　wet　agarose　stamping　technique　and　microcontact　printing　technique.　Because　of　the　specific　reaction　environments　and　diffusion　of　HNO2　confined　in　agarose　stamp,　the　reaction　of　amino　groups　in　the　edge　of　the　strip　pattern　was　much　more　intense　than　other　areas.　The　modified　amino　groups　in　the　edge　areas　show　higher　affinity　to　Au-NPs　than　other　areas,　consequently,　edge　enriched　Au-NPs　patterns　were　observed　after　the　self-assembly　of　Au-NPs.　A　＂cylindrical　droplet＂　model,　in　a　manner　analogous　to　＂coffee-ring＂　effect,　was　proposed　to　describe　the　diffusion　of　HNO2　from　the　bulk　to　the　edge　in　agarose　stamp.　By　using　such　density　varied　Au-NPs　patterns　as　templates　for　the　growth　of　ZnO　nanorods,　we　observed　high　density　of　Au-NPs　resulting　in　high　density　and　highly　(0　0　1)　oriented　ZnO　nanorods.　In　contrast,　sparse　and　non-oriented　ZnO　nanorods　were　grew　on　low　density　of　Au-NPs　areas.　Our　findings　might　open　new　routes　for　the　fabrication　of　gradient　patterns　and　extend　applications　of　Au-NPs　patterns　in　surface　enhanced　Raman　scattering　and　catalysis.