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author:

Xu, Huo (Xu, Huo.) [1] | Zhang, Yafeng (Zhang, Yafeng.) [2] | Zhang, Shuxin (Zhang, Shuxin.) [3] | Sun, Mengze (Sun, Mengze.) [4] | Li, Weihong (Li, Weihong.) [5] | Jiang, Yifan (Jiang, Yifan.) [6] | Wu, Zai-Sheng (Wu, Zai-Sheng.) [7] (Scholars:吴再生)

Indexed by:

EI Scopus SCIE

Abstract:

MicroRNAs (miRNAs) play an important role in the regulation of various biological processes and have been used as potential biomarkers for biomedical research and clinical diagnosis. Here, nicking-mediated rolling circle amplification (N-RCA)/symmetric isothermal circular strand-displacement amplification (S-SDA)-integrated combined cascade amplification (rs-CCA) was proposed for let-7a miRNA detection. Via introducing a palindromic fragment-integrated recognition sites for nicking endonuclease Nt.AlWI into the padlock probe, the hybridization of target miRNA can induce N-RCA and continuously generate the nicked fragments (NFs). Because the released NFs each have a palindromic sequence and daughter nicking site at the 3' end, they hybridize with each other, followed by alternately extension by polymerase and nicking by endonuclease. This leads to S-SDA effect, and the released single-stranded products in turn hybridize with NFs, accomplishing the rs-CCA process. When the Sybr Green I dyes intercalate into double-stranded DNA products, the amplified fluorescence signal is achieved. Thus, the target miRNA can be detected down to 5 pM. Importantly, the rs-CCA system is capable of distinguishing the single base difference between target miRNAs, indicating the high sequence specificity. Moreover, its cpotential application in disease diagnosis was demonstrated via detecting target miRNA in complex biological matrix and analyzing the total RNAs extracted from HeLa cells. As a proof-of-concept building, the impressive rs-CCA scheme is expected to provide a valuable insight into constructing powerful signal amplification strategies via sophisticated combination of biotechnologies available for nucleic acid manipulation and significantly benefit biomedical research and disease diagnostics. (c) 2018 Elsevier B.V. All rights reserved.

Keyword:

amplification (N-RCA) Combined cascade amplification (CCA) miRNA Nicking-mediated rolling circle Palindromic fragment Symmetric isothermal circular strand-displacement amplification (S-SDA)

Community:

  • [ 1 ] [Xu, Huo]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 2 ] [Zhang, Yafeng]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 3 ] [Zhang, Shuxin]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 4 ] [Sun, Mengze]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 5 ] [Li, Weihong]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 6 ] [Jiang, Yifan]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 7 ] [Wu, Zai-Sheng]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China

Reprint 's Address:

  • 吴再生

    [Wu, Zai-Sheng]Fuzhou Univ, Pharmaceut Photocatalysis State Key Lab Photocata, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Canc Metastasis Alert & Prevent Ctr,Coll Chem, Fuzhou 350116, Fujian, Peoples R China

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Source :

ANALYTICA CHIMICA ACTA

ISSN: 0003-2670

Year: 2019

Volume: 1047

Page: 172-178

5 . 9 7 7

JCR@2019

5 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:184

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 47

SCOPUS Cited Count: 48

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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