Mid-story　isolation　eccentric　structures　with　tower-podium　are　examples　of　vertically　irregular　structures.　Its　seismic　performance　and　the　related　mechanisms,　however,　have　not　been　experimentally　examined.　In　this　study,　a　representative　model　of　a　practical　mid-story　isolation　eccentric　structure　with　a　large　podium　was　fabricated,　tested,　and　simulated　to　investigate　its　seismic　response　under　different　earthquakes.　The　results　were　then　compared　to　those　of　an　anti-seismic　model　without　the　isolation　layer.　The　results　show　that　(a)　the　mid-story　isolation　eccentric　structure　with　tower-podium　has　the　similar　characteristics　of　seismic　performance　with　the　regular　story　isolation　structure;　(b)　as　the　peak　acceleration　value　increases,　the　podium　could　quickly　enter　the　elastoplastic　state　and　reach　the　medium　damage　level.　The　damage　extent　in　the　second　layer　of　the　podium　is　larger　than　that　in　its　first　layer.　It　is　thus　recommended　that　the　structural　components　of　the　podium　be　strengthened,　and　the　elastoplastic　displacement　angle　limit　of　the　podium　be　increased.　The　modeling　results　also　indicate　the　torsional　effects　of　such　eccentric　structure　are　effectively　inhibited.　Our　study　enhances　the　understanding　of　the　mid-story　isolation　eccentric　structure　with　tower-podium　and　pioneers　a　guide　to　the　seismic　design　of　such　a　structure.