Herein,　we　report　a　nongenetic　and　real-time　approach　for　imaging　protein　dimerization　on　living　cell　surfaces　by　aptamer　recognition　and　proximity-induced　DNA　assembly.　We　use　the　aptamer　specific　for　the　receptor　monomer　as　a　recognition　probe.　When　receptor　dimerization　occurs,　the　dimeric　receptors　bring　two　aptamer　probes　into　close　proximity,　thereby　triggering　dynamic　DNA　assembly.　The　proposed　approach　was　successfully　applied　to　visualize　dimerization　of　Met　receptor　and　transforming　growth　factor-beta　type　II　receptor.　This　approach　allows　us　to　image　the　two　states　(monomer/dimer)　of　a　receptor　protein　on　living　cell　surfaces　in　real　time,　opening　a　universal　method　for　further　investigation　of　protein　dimerization　and　the　corresponding　activation　processes　in　signal　transduction.