Horizontal　genes　transfer　(HGT)　plays　an　important　role　in　the　dissemination　of　antibiotic　resistance　genes　(ARGs)　in　the　environment.　However,　the　mechanisms　of　HGT　of　ARGs　under　the　influence　of　antibiotics　in　sub-MIC　remain　rarely　explored.　Moreover,　given　its　collective　nature,　HGT　was　considered　to　be　relative　to　quorum　sensing　(QS)　system.　To　investigate　whether　QS　has　any　impact　on　horizontal　gene　transfer　of　ARGs,　experiments　were　conducted　to　determine　the　conjugative　efficiency　of　plasmid　RP4　on　Escherichia　coli　(E.coli)　under　the　influences　of　tetracyclines　(TCs),　quorum　sensing　autoinducers　(AIs)　and　quorum　sensing　inhibitors　(QSIs).　The　results　indicated　that　the　sub-MIC　TCs　could　facilitate　the　conjugative　transfer　of　RP4,　a　process　which　could　be　enhanced　by　AIs　but　inhibited　by　QSIs.　This　study　demonstrated　the　roles　that　QS　played　in　the　dissemination　of　ARGs,　and　provided　theoretical　insights　into　the　mechanism　of　HGT　of　ARGs　in　the　environment.