Purpose:　Biomimetic　mineralized　composite　scaffolds　are　widely　used　as　natural　bone　substitute　materials　in　tissue　engineering　by　inducing　and　assembling　bonelike　apatite.　In　this　study,　the　single　lamellar　structure　of　graphene　oxide　(GO)　powder　was　prepared　via　an　improved　Hummers＇　method.　Methods:　To　better　mimic　natural　bone,　the　collagen　(COL)/Nano-hydroxyapatite　(nHA)/graphene　oxide　(GO)　composite　material　was　prepared　by　simulated　body　fluid　(SBF)　method　using　COL/GO　as　a　matrix　template.　Hydroxyapatite　(HA)　with　calcium　ion　deficiency　was　achieved　via　biomimetic　mineralization,　and　it　had　properties　closer　to　those　of　natural　bone　than　pure　HA　has.　Results:　The　mineralized　COL/nHA/GO　composites　exhibited　loose　porous　structures,　and　the　connectivity　of　the　holes　was　good　and　thus　beneficial　to　the　exchange　of　nutrients　and　excreted　metabolites.　Conculsions:　Antibacterial　and　MTT　experiment　confirmed　that　the　COL/nHA/GO　composite　material　had　excellent　antibacterial　property　and　biocompatibility.　Hence,　these　results　strongly　suggested　the　mineralized　COL/nHA/GO　composite　is　a　good　candidate　biomaterial　to　be　applied　in　bone　tissue　engineering.