Bisphenol　A　(BPA),　a　typical　endocrine　disruptor,　is　widely　used　as　a　key　monomer　in　the　packaging　industry.　Residual　monomer　can　transfer　from　the　package　material　to　the　food　and　thereby　pose　a　risk　to　the　health　of　the　consumer,　so　determination　of　BPA　migration　is　highly　important　for　food　safety　control.　In　this　study,　a　simple　but　sensitive　electrochemiluminescence　(ECL)　biosensor,　which　combines　the　characteristics　of　high　selectivity　of　an　aptamer　and　high　sensitivity　of　ECL,　has　been　developed　to　detect　BPA　from　package　materials.　The　aptamer　was　immobilized　on　a　gold　electrode　surface　through　Au-S　interaction.　The　aptamer　was　then　hybridized　with　complementary　DNA　(CDNA)　to　form　double-stranded　DNA　(dsDNA).　Ru(phen)(3)　(2+)　can　intercalate　into　the　grooves　of　dsDNA　and　acts　as　an　ECL　indicator;　high　ECL　intensity　can　therefore　be　detected　from　the　electrode　surface.　In　the　presence　of　BPA,　which　can　competitively　bind　with　the　aptamer　owing　to　their　high　affinity,　Ru(phen)(3)　(2+)　is　released　from　the　electrode　surface　and　the　ECL　of　the　system　is　decreased.　The　decreasing　ECL　signal　has　a　linear　relationship　with　BPA　in　the　range　of　0.1-100　pM　with　a　detection　limit　of　0.076　pM.　The　developed　biosensor　has　been　applied　to　detect　migration　of　BPA　from　different　categories　of　canned　drink　with　satisfactory　results.