Aflatoxin　B-1　(AFB(1))　monitoring　has　attracted　extensive　attention　because　food　safety　is　a　worldwide　public　health　problem.　Herein,　we　design　a　novel　simultaneously　visual　and　photoelectrochemical　(PEC)　immunosensing　system　for　rapid　sensitive　detection　of　AFB(1)　in　foodstuff　The　immunoreaction　was　carried　out　on　anti-AFB,　antibody-modified　magnetic　beads　by　using　glucose　oxidase　(GOX)-labeled　AFB(1-)bovine　serum　albumin　(AFB(1)-BSA)　conjugates　as　the　tags　with　a　competitive-type　immunoassay　format,　while　the　visual　and　PEC　evaluation　was　performed　via　carbon　quantum　dots　(CQDs)-functionalized　MnO2　nanosheets.　Accompanying,　the　formation　of　immunocomplexes,　the　carried　GOx　initially　oxidized　the　substrate　(glucose)　for　the　generation　of　H2O2,　which　reduced/etched　MnO2　nanosheets　into　Mn2+-ions,　thereby　resulting　in　the　dissociation　of　CQDs　from　the　electrode.　Within　the　applied　potentials,　the　photocurrent　of　MnO(2-)CQDs-modified　electrode　decreased　with　the　increasing　H2O2　level　in　the　detection　cell.　Meanwhile,　a　visual　detection　could　be　performed　according　to　the　change　in　the　color　of　MnO2-CQDs-coated　electrode.　To　elaborate,　this　system　was　aggregated　into　a　high　-throughput　microfluidic　device　to　construct　a　semiautomatic　detection　cell.　Under　optimal　conditions,　the　photocurrent　increased　with　the　increasing　target　AFB1　within　a　dynamic　working　range　from　0.01　to　20　ng　mL(-1)　with　a　limit　of　detection　(LOD)　of　2.1　pg　mL(-1)　(ppt).　The　developed　immunoassay　exhibited　good　reproducibility　and　acceptable　accuracy.　In　addition,　the　method　accuracy　relative　to　AFB1　ELISA　kit　was　evaluated　for　analyzing　naturally　contaminated　or　spiked　peanut　samples,　giving　the　well-matched　results　between　two　methods.　Although　our　strategy-was　focused　on　the　detection　of　target　AFB1,　it　is　easily　extended　to　screen　other　small　molecules　or　mycotoxins,　thereby　representing　a　versatile　immunosensing　scheme.