A　stable,　sensitive　and　low-reagent　consumption　method　for　the　quantification　of　trace　neurotoxin　domoic　acid　(DA)　was　presented　by　pressurized　capillary　electrochromatography　(pCEC)　with　laser-induced　fluorescence　(LIF)　detection.　Migration　behaviours　of　DA　were　studied　under　different　separation　modes　such　as　pCEC　and　capillary　HPLC.　Separation　parameters　including　the　mobile　phase,　applied　voltage,　supplementary　pressure　and　sample　loading　rate　were　investigated.　Double　modes　including　electrophoretic　migration　and　chromatographic　retention　were　employed　and　an　efficient　separation　of　DA　was　achieved　with　a　flow　of　1.2　mu　L　min(-1)　in　a　capillary　column.　Sensitive　analysis　of　DA　was　achieved　with　a　detection　limit　of　DA　in　shellfish　tissue　extracts　as　low　as　10　ng　mL(-1)　(equivalent　to　15　ng　DA　per　g,　wet　weight).　The　stability　and　repeatability　of　pCEC-LIF　was　evaluated　and　a　good　result　was　gained　with　RSDs　(n　=　3)　for　the　retention　time　and　peak　area　of　DA　less　than　0.5%　and　2.0%,　respectively.　Applied　to　shellfish　samples,　the　recoveries　were　satisfactory　and　the　variation　of　the　quantification　of　DA　was　acceptable　when　compared　with　the　HPLC-MS/MS　method.