GST-TAT-SOD,　a　cell-permeable　bifunctional　antioxidant　enzyme,　is　a　potential　selective　radioprotector.　This　study　aimed　to　investigate　the　cytoprotective　activity　of　GST-TAT-SOD　against　cisplatin-induced　damage.　The　current　study　showed　that　cisplatin　induced　the　formation　of　reactive　oxygen　species　in　normal　L-02　cells.　GST-TAT-SOD　(　2000　U/mL)　executed　its　antioxidant　role　by　directly　scavenging　excess　intracellular　free　radicals　and　augmenting　cellular　antioxidant　defense　such　as　reducing　MDA　level,　enhancing　the　SOD　activity,　GST　activity,　and　T-AOC.　Thus,　it　suppressed　the　growth　inhibition　and　apoptosis　of　cisplatin-treated　normal　cells.　Meanwhile,　the　growth　inhibition　of　tumor　cells　(SMMC-7721)　caused　by　cisplatin　was　unaffected　by　GST-TAT-SOD　pretreatment.　GST-SOD,　as　a　comparison,　seemed　to　be　powerless　for　related　indicators　as　it　could　not　enter　into　cells　without　cell-permeating　peptide.　These　results　suggest　that　GST-TAT-SOD　might　be　a　potential　cytoprotective　agent　for　cisplatin-induced　side　effects.