Chirality　is　one　of　the　essential　features　in　our　living　life　and　exerts　a　wide　variety　of　applications　in　enantio-adsorption/separation.　However,　the　mechanism　between　chirality　and　enantio-adsorption/separation　is　very　significant　in　homochiral　porous　materials;　in　particular,　the　understanding　of　the　relationship　between　crystalline　orientations　and　chiral　behavior　is　a　challenging　but　important　mechanism.　In　this　work,　homochiral　porous　crystalline　metal　organic　framework　(MOF)　materials　were　grown　on　hydroxyl-　and　carboxyl-functionalized　substrates,　resulting　in　homochiral　porous　thin　films　with　different　orientations.　The　enantioselectivity　and　adsorption　rates　in　two　different　oriented　homochiral　porous　thin　films　were　studied　by　using　gas-phase　quartz　crystal　microbalance　(QCM)　experiment　of　chiral　probe　molecules.　The　different　mass　uptake　and　time　constant　showed　that　the　chiral　behavior　can　be　obviously　influenced　by　the　crystalline　orientations　on　the　same　homochiral　porous　thin　films.　This　study　will　not　only　offer　a　good　model　to　understand　the　mechanism　of　chiral　behavior　in　homochiral　porous　materials　but　also　provide　guidance　for　developing　new　homochiral-oriented　porous　thin　films　with　high　enantioselectivity　or　enantioseparation.