The　authors　report　on　a　new　electrochemical　aptasensing　strategy　for　the　determination　of　adenosine　-　5＇-triphosphate　(ATP)　at　picomolar　levels.　First,　manganese　dioxide　(MnO2)　nanosheets　with　an　average　size　of　similar　to　70　nm　were　synthesized　via　a　hot-injection　method　on　the　basis　of　reaction　between　potassium　permanganate　and　the　cationic　detergent　cetyltrimethylammonium　bromide.　The　resulting　MnO2　nanosheets　were　then　immobilized　onto　a　pretreated　screen-printed　carbon　electrode　which　readily　binds　the　ferrocene-labeled　ATP　aptamer　through　the　van　der　Waals　force　between　the　nucleobases　and　the　basal　plane　of　the　nanoflakes.　The　immobilized　ferrocene-aptamer　conjugates　activates　the　electrical　contact　with　the　electrode　and　produces　a　strong　signal　in　the　potentials　scanned　(0.0　to　1.0　V　vs.　Ag/AgCl).　Upon　addition　of　ATP,　it　will　react　with　the　aptamer　and　cause　the　dissociation　of　the　ferrocene-aptamer　from　the　nanosheets,　this　resulting　in　a　decrease　in　the　electrical　signal.　Under　optimal　conditions,　this　platform　exhibits　a　detection　limit　as　low　as　0.32　nM　of　ATP.　The　repeatability　and　intermediate　precision　is　below　10.7　%　at　a　10　nM　concentration　level.　The　method　was　applied　to　analyze　blank　fetal　calf　serum　spiked　with　ATP,　and　the　recoveries　(at　3　concentration　levels)　ranged　between　91.3　and　118　%.　This　detection　scheme　is　rapid,　simple,　cost-effective,　and　does　not　require　extensive　sample　preparation　or　multiple　washing　steps.