Horseradish　peroxidase　shows　potential　biological　and　environmental　applications　on　the　removal　of　phenolic　compounds.　In　the　present　study,　the　HRP-immobilized　beads　were　synthesized　to　detect　the　efficiency　of　the　removal　of　phenol　at　optimum　pH　and　H2O2　concentration.　Comparative　in　vitro　cytotoxicity　of　phenol/treated　solutions　were　evaluated　in　HeLa,　HepG2　and　mcf-7　cells　by　using　MTT　method　along　with　flow　cytometry　study　for　cell　viability　and　cell　cycle　distributions.　The　results　showed　that　the　toxicity　of　phenol　solutions　were　greatly　reduced　after　treated　by　HRP-immobilized　beads,　and　phenol　could　lead　to　deactivate　of　cells　in　the　S　phase　and　preventing　them　from　going　into　the　G2/M　checkpoint.　In　addition,　molecular　docking　study　showed　that　phenol　was　a　valid　inhibitor　for　the　cyclin　E　in　the　cell　cycle　and　cell　metabolism.　Thereby,　we　established　a　suitable　strategy　with　promising　application　for　efficient　harmless　removal　of　phenol,　which　significantly　decreased　the　cytotoxic　impacts　of　phenol.　(C)　2016　Elsevier　Inc.　All　rights　reserved.