Enhancing　catalytic　activity　of　multi-enzyme　in　vitro　through　substrate　channeling　effect　is　promising　yet　challenging.　Herein,　conjugated　microporous　polymers　(CMPs)-scaffolded　integrated　enzyme　cascade　systems　(I-ECSs)　are　constructed　through　co-entrapping　glucose　oxidase　(GOx)　and　horseradish　peroxidase　(HRP),　in　which　hydrogen　peroxide　(H2O2)　is　the　intermediate　product.　The　interplay　of　low-resistance　mass　transfer　pathway　and　appropriate　pore　wall-H2O2　interactions　facilitates　the　directed　transfer　of　H2O2,　resulting　in　2.4-fold　and　5.0-fold　elevation　in　catalytic　activity　compared　to　free　ECSs　and　separated　ECSs,　respectively.　The　substrate　channeling　effect　could　be　regulated　by　altering　the　mass　ratio　of　GOx　to　HRP.　Besides,　I-ECSs　demonstrate　excellent　stabilities　in　harsh　environments　and　multiple　recycling.　(c)　2024,　Dalian　Institute　of　Chemical　Physics,　Chinese　Academy　of　Sciences.　Published　by　Elsevier　B.V.　All　rights　reserved.