A　new　gold　particles　modified　heated　copper　disk　electrode　(Au-HCuDE)　with　direct　current　was　fabricated.　The　hemin/G-quadruplex　horseradish　peroxidase-mimicking　DNAzyme　(HRP-DNAzyme)　was　self-assembled　on　the　heated　electrode　and　resulted　in　a　new　biosensor　denoted　as　HRP-DNAzyme/AuHCuDE.　By　controlling　the　temperature　of　the　surface　of　the　electrode,　the　dramatic　temperature　effect　on　the　electrocatalytic　reduction　of　H2O2　at　HRP-DNAzyme/Au-HCuDE　sensing　platform　was　demonstrated.　This　electrocatalytic　activity　of　HRP-DNAzyme　was　enhanced　with　electrode　temperature　elevated.　This　method　was　thus　preliminarily　used　to　develop　an　electrochemical　biosensor　for　highly　sensitive　detection　of　H2O2.　A　detection　limit　of　1.6　x　10(-7)　M　could　be　obtained　(S/N=3)　with　an　electrode　temperature　of　50　degrees　C,　which　was　more　than　one　magnitude　lower　than　that　at　electrode　temperature　of　0　degrees　C.　This　heated　electrochemical　biosensor　shows　many　merits　such　as　easy　fabrication　and　simple　heating　equipment,　low　cost,　high　thermal　stability,　and　high　sensitivity　and　good　reproducibility.　(C)　2015　Elsevier　B.V.　All　rights　reserved.