A　novel.　biocompatible　sensing　strategy　based　on　graphene　and　chitosan　composite　film　for　immobilizing　the　hemoglobin　protein　was　firstly　adopted.　The　direct　electron　transfer　and　bioelectrocatalytic　activity　of　hemoglobin　after　incorporation　into　the　composite　film　were　investigated.　A　pair　of　reversible　redox　waves　of　hemoglobin　was　appeared,　and　hemoglobin　could　exhibit　its　bioelectrocatalytic　activity　toward　H2O2　in　a　long　term.　Such　results　indicated　that　graphene　and　chitosan　composite　could　be　a　friendly　biocompatible　interface　for　immobilizing　biomolecules　and　keeping　their　native　structure.　Furthermore,　the　appearance　of　graphene　in　the　composite　film　could　facilitate　the　electron　transfer　between　matrix　and　the　electroactive　center　of　hemoglobin.　Hence,　this　graphene　and　chitosan　based　protocol　would　be　a　promising　platform　for　protein　immobilization　and　biosensor　preparation.　(C)　2010　Elsevier　BM.　All　rights　reserved.