In　this　study,　the　effects　of　the　type　and　concentration　of　nitrogen　sources　on　the　cell　growth　and　lutein　content　of　an　isolated　microalga　Scenedesmus　obliquus　FSP-3　were　investigated.　With　batch　culture,　the　highest　lutein　content　(4.61　mg/g)　and　lutein　productivity　(4.35　mg/L/day)　were　obtained　when　using　8.0　mM　calcium　nitrate　as　the　nitrogen　source.　With　this　best　nitrogen　source　condition,　the　microalgae　cultivation　was　performed　using　two　bioreactor　strategies　(namely,　semi-continuous　and　two-stage　operations)　to　further　enhance　the　lutein　content　and　productivity.　Using　semi-continuous　operation　with　a　10%　medium　replacement　ratio　could　obtain　the　highest　biomass　productivity　(1304.8　mg/L/day)　and　lutein　productivity　(6.01　mg/L/day).　This　performance　is　better　than　most　related　studies.　(C)　2014　Elsevier　Ltd.　All　rights　reserved.