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author:

Liu, Ting (Liu, Ting.) [1] | Chen, Xian (Chen, Xian.) [2] | Hong, Cheng-Yi (Hong, Cheng-Yi.) [3] | Xu, Xiao-Ping (Xu, Xiao-Ping.) [4] (Scholars:许小平) | Yang, Huang-Hao (Yang, Huang-Hao.) [5] (Scholars:杨黄浩)

Indexed by:

Scopus SCIE

Abstract:

Electrochemiluminescence (ECL) integrates the advantages of electrochemical detection and chemiluminescent techniques. The method has received particular attention because it is highly sensitive and selective, has a wide linear range but low reagent costs. The use of nanomaterials with their unique physical and chemical properties has led to new kinds of biosensors that exhibit high sensitivity and stability. Compared to other nanomaterials, DNA nanostructures are more biocompatible, more hydrophilic, and thus less prone to nonspecific adsorption onto the electrode surface. We describe here a label-free and ultrasensitive ECL biosensor for detecting a cancer-associated microRNA at a femtomolar level. We have designed two auxiliary probes that cause the formation of a long-range self-assembly in the form of a mu m-long 1-dimensional DNA concatamer. These can be used as carriers for signal amplification. The intercalation of the ECL probe Ru(phen)(3) (2+) into the grooves of the concatamers leads to a substantial increase in ECL intensity. This amplified sensor shows high selectivity for discriminating complementary target and other mismatched RNAs. The biosensor enables the quantification of the expression of microRNA-21 in MCF-7 cells. It also displays very low limits of detection and provides an alternative approach for the detection of RNA or DNA detection in diagnostics and gene analysis.

Keyword:

DNA concatamers Electrochemiluminescence MicroRNA Nanomaterials

Community:

  • [ 1 ] [Liu, Ting]Fuzhou Univ, Coll Chem & Chem Engn, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Key Lab Anal & Detect Technol Food Safety,MOE, Fuzhou 350108, Peoples R China
  • [ 2 ] [Chen, Xian]Fuzhou Univ, Coll Chem & Chem Engn, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Key Lab Anal & Detect Technol Food Safety,MOE, Fuzhou 350108, Peoples R China
  • [ 3 ] [Hong, Cheng-Yi]Fuzhou Univ, Coll Chem & Chem Engn, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Key Lab Anal & Detect Technol Food Safety,MOE, Fuzhou 350108, Peoples R China
  • [ 4 ] [Xu, Xiao-Ping]Fuzhou Univ, Coll Chem & Chem Engn, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Key Lab Anal & Detect Technol Food Safety,MOE, Fuzhou 350108, Peoples R China
  • [ 5 ] [Yang, Huang-Hao]Fuzhou Univ, Coll Chem & Chem Engn, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Key Lab Anal & Detect Technol Food Safety,MOE, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • 许小平

    [Xu, Xiao-Ping]Fuzhou Univ, Coll Chem & Chem Engn, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Key Lab Anal & Detect Technol Food Safety,MOE, Fuzhou 350108, Peoples R China

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Source :

MICROCHIMICA ACTA

ISSN: 0026-3672

Year: 2014

Issue: 7-8

Volume: 181

Page: 731-736

3 . 7 4 1

JCR@2014

5 . 4 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:268

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 30

SCOPUS Cited Count: 32

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 6

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