Epidermal　growth　factor　receptor　(EGFR)　that　has　been　over-expressed　in　many　different　types　of　cancers　has　become　logical　targets　for　both　diagnosis　and　therapy.　Herein,　we　report　an　electrochemical　peptide-based　biosensor　for　the　detection　of　EGFR,　in　which　ferrocene　moiety　modified　peptide　ligands　that　are　specific　to　EGFR　were　immobilized　on　gold　electrode　through　Au-S　interaction.　Upon　the　binding　of　peptide　ligands　with　EGFR,　complex　formation　changes　occurred　on　the　electrode　surface,　which　resulted　in　current　increase.　The　amplitude　of　the　current　increase　displayed　a　linear　relationship　with　logarithm　of　the　EGFR　concentrations　in　the　range　of　1.0　x　10(-6)-1.0　x　10(-10)　g　L-1,　with　a　detection　limit　of　3.7　x　10(-11)　g　L-1.　The　proposed　method　has　been　applied　for　determination　of　EGFR　in　diluted　human　serum　samples　with　satisfied　results.　(C)　2013　Elsevier　Ltd.　All　rights　reserved.