This　work　aims　to　explore　the　application　of　silver　nanoparticle-based　surface-enhanced　Raman　scattering　(SERS)　for　nasopharyngeal　carcinoma　cell　line　CNE2＇s　DNA　analysis　after　X-ray　radiation.　The　cells　are　separated　into　control　group　and　radiated　groups　with　different　dose　of　6,　10,　15　and　20Gy.　The　results　show　that　after　radiation　(6,　10,　15　and　20Gy),　the　DNA　of　radiated　CNE2　have　changed　after　72h　of　cell　incubation.　Principal　components　analysis　is　employed　for　significant　differences　and　the　DNA　extracted　after　72h　of　incubation　show　significant　divisions　from　control　group.　Moreover,　a　classifier　based　on　support　vector　machines　shows　high　classification　accuracy　between　DNA　extracted　after　72h　of　incubation　and　control　group.　In　conclusion,　this　study　first　reveals　SERS　characteristics　of　CNE2＇s　DNA　under　different　dose　of　X-ray　radiation,　and　the　final　results　may　do　favor　to　make　known　the　mechanism　of　X-ray　radiation　interacting　with　tumor.　Copyright　(c)　2013　John　Wiley　&　Sons,　Ltd.