An　easy,　rapid　method　for　the　simultaneous　determination　of　isotetrandrine,　curine,　sinactine,　sinomenine　and　magnoflorine　in　Sinomenium　acutum　was　developed　by　a　capillary　electrophoresis　(CE)　method　with　field-amplified　sample　stacking　(FASS).　Parameters　affecting　CE　separation　and　FASS　performance　were　all　carefully　investigated　to　obtain　the　best　sensitivity　with　the　highest　speed.　Under　the　optimized　conditions:　UV　detection　at　214　nm,　separation　voltage　25　kV,　40　mM　sodium　citrate-citric　acid　(pH　3.5)　as　running　buffer,　the　detection　sensitivity　was　from　4.8　x　10(-9)　M　to　1.0　x　10(-8)　M.　This　resulted　in　20-100-fold　improvement　in　concentration　sensitivity　relative　to　conventional　capillary　electrophoresis　methods.　The　relative　standard　deviation　(RSD)　of　the　peak　area　ratios　for　repeatability　(intraday,　n　=　5)　and　intermediate　precision　(interday,　n　=　5)　were　lower　than　4.1%　and　5.4%,　respectively.　Finally,　the　developed　method　was　successfully　applied　to　qualitatively　and　quantitatively　analyze　five　alkaloids　in　Sinomenium　acutum,　with　the　recovery　ranging　from　75.5　to　102.2%.