A　highly　porous　three-dimensional　sensing　interface　was　achieved　on　glassy　carbon　(GCE)　by　employing　ordered　mesoporous　carbon　(OMC)　and　polyaniline　(PANI).　Compared　with　the　cathodic　electrochemiluminescence　(ECL)　of　luminol　at　bare　glassy　carbon　electrode,　a　stable　and　intense　cathodic　ECL　emission　of　luminol　over　-0.5　V　which　related　to　the　reduction　of　dissolved　oxygen　in　solution　was　triggered　at　OMC　and　PANI　constructed　ECL　sensing　platform.　The　influence　factors　affecting　the　electrode　configuration　such　as　the　concentration　of　OMC,　the　dripping　amount　of　OMC,　the　polymerization　cycles　of　PANI　and　the　pH　value　of　buffer　solution　on　cathodic　ECL　intensity　of　luminol　were　investigated　in　detail.　The　PANI/CMK　composite　modified　electrode　exhibited　an　effective　ECL　platform　for　cathodic　ECL　of　luminol　due　to　its　attractive　features,　such　as　excellent　electrical　conductivity,　extremely　well-ordered　pore　structure　and　high　specific　pore　volume.　The　possible　ECL　mechanism　was　discussed　according　to　the　presented　experimental　results.　(C)　2012　Published　by　Elsevier　Ltd.