Citrinin　(CIT)-protein　artificial　antigen　was　prepared　by　conjugating　CIT　with　keyhole　limpet　hemocyanin　by　1,4-butanediol　diglycidyl　ether.　By　immunization　and　fusion,　a　hybridoma　cell　line　named　K2-F3,　which　stably　secreted　the　monoclonal　antibody　(McAb)　against　CIT　was　obtained.　The　titer　of　the　CIT-specific　McAbs　purified　by　affinity　chromatography　came　to　1:1.38　x　10(5).　The　indirect　competitive　enzyme-linked　immunosorbent　assay　(ELISA)　showed　that　the　detection　limit　of　CIT　was　0.01　ng/mL,　with　a　good　linearity　ranging　from　0.01　to　1.0　ng/mL.　The　McAb　was　highly　specific　and　cross-reactivity　rate　was　less　than　0.01%.　The　data　showed　that　the　ELISA　developed　could　accurately　determine　CIT　in　the　real　contaminated　red　yeast　rice　samples.　The　systematic　error　was　low　with　the　coefficient　of　variation　from　2.91　to　8.53%　by　the　ELISA.