A　polymethacrylate-based　molecularly　imprinted　monolithic　column　bearing　mixed　functional　monomers,　using　non-covalent　imprinting　approach,　was　designed　for　the　rapid　separation　of　nitroimidazole　compounds.　The　new　monolithic　column　has　been　prepared　via　simple　in　situ　polymerization　of　2-hydroxyethyl　methacrylate,　dimethylaminoethyl　methacrylate　and　ethylene　dimethacrylate,　using　(S)-ornidazole　((S)-ONZ)　as　template　in　a　binary　porogenic　mixture　consisting　of　toluene　and　dodecanol.　The　composition　of　the　polymerization　mixture　was　systematically　altered　and　optimized　by　altering　the　amount　of　monomers　as　well　as　the　composition　of　the　porogenic　solvent.　The　column　performance　was　evaluated　in　pressure-assisted　CEC　mode.　Separation　conditions　such　as　pH,　voltage,　amount　of　organic　modifier　and　salt　concentration　were　studied.　The　optimized　monolithic　column　resulted　in　excellent　separation　of　a　group　of　structurally　related　nitroimidazole　drugs　within　10　min　in　isocratic　elution　condition.　Column　efficiencies　of　99　000,　80000,　103　000,　60　000　and　99　000　plates/m　were　obtained　for　metronidazole,　secnidazole,　ronidazole,　tinidazole　and　dimetridazole,　respectively.　Parallel　experiments　were　carried　out　using　molecularly　imprinted　and　non-imprinted　capillary　columns.　The　separation　might　be　the　result　of　combined　effects　including　hydrophobic,　hydrogen　bonding　and　the　imprinting　cavities　on　the　(S)-ONZ-imprinted　monolithic　column.