An　ethanol　biosensor,　based　on　the　electrogenerated　chemiluminescence　of　Ru(bpy)(3)(2+)-doped　silica　nanoparticles　(RuSiNPs),　was　investigated　in　this　study.　The　biosensor　was　a　modified　glassy　carbon　electrode,　where　alcohol　dehydrogenase　was　crosslinked　to　RuSiNPs,　and　then　immobilized　on　the　electrode　surface　using　chitosan.　The　results　indicated　that　the　biosensor　exhibited　excellent　performance　during　ethanol　determination　with　a　wide　linear　range　(10(-7)　to　10(-2)　M),　low　detection　limit　(5.0　x　10(-8)　M)　and　good　stability.　(C)　2009　Elsevier　B.V.　All　rights　reserved.