A　novel　graphite/poly(methyl　methacrylate)　(graphite/PMMA)　electrode　was　prepared　in　this　paper.　it　was　found　that　the　developed　polymer　graphite　paste　electrode　has　some　advantages　in　electrochemistry　and　electrochemiluminescence　(ECL),　such　as　high　sensitivity,　good　reproducibility,　quick　and　wide　linear　range　of　response　to　some　biomolecules.　The　ECL　behavior　of　luminol　has　been　investigated　in　detail　at　the　graphite/PMMA　electrode,　and　vitamin　C　was　found　to　be　able　to　inhibit　this　ECL　system.　Based　on　which　an　inhibited　ECL　detection　method　has　been　developed　for　determination　of　vitamin　C　in　this　paper.　The　proposed　method　exhibited　good　reproducibility,　wide-range　linearity,　high　sensitivity　and　stability　with　a　detection　limit　of　8.3　x　10(-9)　mol　L-1　(signal-to-noise　ratio　=　3)　and　linear　response　range　of　2.5　x　10(-8)　-　1.0　x　10(-4)　mol　L-1.　The　relative　standard　deviation　was　2.3%　for　5　x　10(-6)　mol　L-1　vitamin　C　(n　=　9).　The　possible　mechanism　for　inhibition　of　luminol　on　graphite/PMMA　electrode　has　also　been　proposed.　(C)　2008　Elsevier　Ltd.　All　rights　reserved.