CZE　with　UV-absorption　detection　has　been　used　for　the　separation　and　determination　of　enkephalin-related　peptides.　The　experimental　conditions,　such　as　pH　and　concentration　of　running　buffer,　applied　voltage,　injection　method,　and　time,　were　investigated　in　detail.　Excellent　separation　efficiency　could　be　obtained　for　ten　enkephalin-related　peptides　with　a　50　mu　m　(ID)　x　58　cm　capillary　using　sodium　dihydrogen　phosphate　as　the　running　buffer　(pH　3.11)　when　20　kV　of　applied　voltage　was　used.　The　concentration　detection　limits　were　found　to　be　in　the　range　of　0.31-1.94　mu　g/mL　(defined　as　S/N　=　3).　The　proposed　method　has　been　applied　to　analyze　the　spiked　cerebrospinal　fluid　(CSF)　sample,　and　the　results　showed　that　CZE　is　a　powerful　technique　for　separation　and　detection　of　the　above　biological　peptides.