The　spherical　lignin　adsorbent　was　prepared　by　applying　reverse-phase　suspension　polymerization　technique　with　alkali　lignin　as　raw　material.　Then　the　spherical　lignin　beads　were　adopted　to　adsorb　the　L-alanine.　And,　the　adsorption　conditions　were　also　optimized.　The　results　indicated　the　adsorption　of　L-alanine　on　the　spherical　lignin　beads　has　been　found　to　be　time,　initial　mass　concentration　and　solution　pH　dependent.　The　equilibrium　adsorption　capacity　could　reach　83.28　mg/g　under　such　conditions　as　120　min　of　the　adsorption　time,　400　mg/L　of　the　initial　mass　concentration　of　L-alanine　aqueous　solution　and　2.0　of　solution　pH.　Moreover,　NH(4)Cl　and　NaCl　also　showed　great　effect　on　the　adsorption　efficiency　when　existed　in　large　amount.　The　adsorption　percentage　decreased　from　83.28%　to　4.35%　with　an　increase　of　salt　concentration　in　aqueous　solutions　among　the　concentration　range　of　0.5　and　1.5　mol/L.　Simultaneously,　L-alanine　could　be　desorbed　with　ammonia　of　1.0　mol/L,　and　the　regeneration　capacity　of　lignin　bead　was　good.