The　objective　of　this　study　was　to　evaluate　the　relative　glycemic　index　of　low　gluten　rice　(W0868)　in　healthy　adult　Beagle　dogs　and　to　comparatively　and　quantitatively　evaluate　the　effect　of　W0868　on　postprandial　blood　glucose　and　insulin　levels,　so　as　to　provide　a　theoretical　reference　for　the　application　and　further　studies　of　low　gluten　rice.　Methods:The　postprandial　blood　glucose　concentration　was　measured　by　an　automatic　biochemical　analyzer,　and　the　postprandial　insulin　content　of　serum　was　measured　by　an　enzyme-linked　immunosorbent　assay　kit.　The　results　showed　that　the　total　cumulative　effect　of　W0868　on　increasing　the　blood　glucose　level　of　Beagle　dogs　was　lower　than　that　of　the　control　diet,　especially　at　postprandial　1　hour.　In　addition,　according　to　the　definition　of　glycemic　index,　the　geometric　mean　value　of　relative　glycemic　index　of　W0868　compared　with　the　control　diet　was　calculated　as　59.33%　with　a　90%　confidence　interval　of　42.56%-82.72%　using　the　area　under　the　plasma　glucose　curve　for　0-120　min　after　baseline　correction.　Therefore,　it　can　be　considered　that　the　rate　and　degree　of　increase　in　blood　glucose　of　the　animals　fed　with　W0868　was　40.67%　lower　than　that　with　the　control　diet　under　the　same　standard　single　ration　feeding　regimen.　Therefore,　the　postprandial　glycemic　index　of　W0868　is　low　in　Beagle　dogs　and　it　can　result　in　smooth　postprandial　glycemic　fluctuations.　©　2020,　China　Food　Publishing　Company.　All　right　reserved.