Rubropunctatin　is　a　naturally　occurring　constituent　of　polyketide　compounds　that　has　great　potential　in　the　development　of　cancer-assisted　chemotherapy.　However,　it　has　certain　shortcomings　such　as　water　insolubility　and　photo　instability　that　limit　its　clinical　application.　In　this　study,　we　constructed　a　Rubropunctatin-loaded　liposome　(R-Liposome)　anticancer　drug　carrier　for　the　first　time.　The　results　indicate　that　R-Liposome　is　water　soluble,　has　spherical　morphology,　great　homogeneity　and　dispersibility　with　high　encapsulation　efficiency　(EE%,　90　±　3.5%)　and　loading　rate　(LR%,　5.60　±　2.5%)　values.　Moreover,　the　carrier　improves　the　photostability,　storage　and　pH　stabilities　of　Rubropunctatin.　The　R-Liposome　also　prolongs　the　release　of　Rubropunctatin,　enhances　the　anticancer　activity　of　Rubropunctatin　and　encourages　the　mechanism　of　Rubropunctatin　to　promote　apoptosis.　Therefore,　liposomal　nanoparticles　have　great　potential　as　drug　delivery　vehicles　of　Rubropunctatin　for　cancer　treatment.　This　journal　is　©　The　Royal　Society　of　Chemistry.