An　analytical　method　was　established　for　rapid　screening　of　16　hormone　residues　in　aquatic　product　by　a　modified　Quick,　Easy,　Cheap,　Effective,　Rugged,　and　Safe　(QuEChERS)　combined　with　ultra　performance　liquid　chromatographyquadrupole　time　of　flight　mass　spectrometry　(UPLC-Q-TOF　MS).　The　homogenized　samples　were　extracted　with　acetonitrile　containing　1%　acetic　acid,　and　the　extract　was　desalted　with　sodium　chloride　and　anhydrous　magnesium　sulfate,　defatted　with　n-hexane　and　then　cleaned-up　on　a　C18column.　The　analytes　were　separated　on　a　BEH　C18column　using　a　mixture　of　0.1%　formic　acid　and　methanol　as　mobile　phase　by　gradient　elution　and　analyzed　by　MS　using　electrospray　ionization(ESI)　in　positive　ionization　mode.　The　linear　range　was　1-100　μg/L　for　all　compounds　with　correlation　coefficients　of　more　than　0.990　6.　The　recoveries　of　the　hormones　at　three　different　spiked　levels　ranged　from　53.6%　to　135.2%,　with　relative　standard　deviations　(RSDs)　of　0.93%　to　10.9%　in　Penaeus　vannamei,　Pseudosciaena　crocea,　grass　carp　and　swimming　crab　matrices.　The　limits　of　detection　(LODs)　(RSN=3)　were　in　the　range　of　0.2-2.7　μg/kg　and　the　limits　of　quantification　(LOQs)　(RSN=　10)　for　the　analytes　were　0.8-8.2　μg/kg.　The　method　is　simple,　rapid,　accurate　and　suitable　for　rapid　screening　of　hormones　in　aquatic　products　samples.　©　2018,　China　Food　Publishing　Company.　All　right　reserved.