Liposomes　are　an　excellent　candidate　component　for　biosensors　to　transduce　and　amplify　detection　signals　due　to　their　outstanding　ability　in　encapsulating　signal　marker　compounds.　However,　the　use　of　liposomes　for　photoelectrochemical　(PEC)　signal　transduction　has　not　yet　been　achieved　due　the　lack　of　appropriate　sensing　strategy.　Herein,　we　report　on　a　novel　liposomes-amplified　PEC　immunoassay　(LAPIA)　method　for　sensitive　HIV-p24　antigen　(p24)　detection　based　on　a　split-type　strategy.　Initially,　liposomes　were　encapsulated　with　alkaline　phosphatase　(ALP)　in　their　hydrophilic　chamber　and　conjugated　with　secondary　antibody　on　the　surface　to　form　the　ALP-encapsulated　liposomes　(ALP-Ls)　based　PEC　signal　label.　Sandwiched　immunoassay　based　on　the　ALP-Ls　label　was　then　carried　out　in　microwell　plate.　Upon　addition　of　tween　20,　the　ALP　molecules　were　released　and　catalyzed　the　hydrolysis　of　ascorbic　acid　2-phosphate　(AA-p)　to　produce　ascorbic　acid　(AA).　The　latter　then　donated　electron　to　the　graphene/g-C3N4　nanohybrids　based　photoelectrode,　arousing　an　increased　photocurrent　signal.　The　separation　of　immunoreaction　step　and　PEC　signal　excitation　(i.e.　split-type)　not　only　enabled　the　realization　of　liposomes　based　amplification　strategy,　but　also　could　eliminate　the　PEC-caused　biomolecules　damage.　The　developed　PEC　method　possessed　a　wide　calibration　range　from　1.0 pg mL−1　to　50 ng mL−1　and　a　low　detection　limit　of　0.63 pg mL−1.　Its　practicability　was　demonstrated　by　assaying　human　serum　samples.　Moreover,　the　universality　of　the　liposomes-amplified　PEC　sensing　strategy　was　also　demonstrated　by　developing　it　into　a　sensitive　microRNA　detection　method.　©　2017　Elsevier　B.V.