Based　on　in-tube　solid-phase　microextraction　(in-tube　SPME)　using　a　hydrophobic　poly　(octadecyl　methacrylate-co-ethylene　dimethacrylate)　[poly　(OMA-co-EDMA)]　monolith,　a　simple　high　performance　liquid　chromatography　(HPLC)　method　has　been　developed　for　detection　of　trans-fatty　acids　(TFAs)　as　their　fatty　acid　methyl　esters　(FAMEs).　The　poly　(OMA-co-EDMA)　monolithic　column　with　high　hydrophobicity　was　specially　prepared　for　simultaneous　microextraction,　pre-separation　and　purification　for　the　analytes.　The　pre-separation　selectivity,　the　extraction　efficiency,　and　the　purification　effect　for　FAMEs　were　investigated　respectively.　Furthermore,　some　operation　parameters　have　been　optimized　in　detail　with　respect　to　satisfactory　extraction　efficiency　of　the　target　compounds.　Under　the　optimized　conditions,　the　enrichment　factors　for　model　FAMEs　were　ranged　from　58.3　to　70.9,　wide　linear　range　(0.01-1.00 mg/kg)　and　low　detection　limits　(LODs)　(3.0-7.1 μg/kg)　were　achieved,　respectively.　In　addition,　recoveries　of　the　method　were　in　the　range　from　83.0　to　106.4%　with　low　relative　standard　deviations　(RSDs)　of　3.2-4.7%　(n = 4)　at　spiking　levels　of　0.05,　0.25　and　0.5 mg/kg,　respectively.　Finally,　the　proposed　method　was　successfully　applied　for　detection　of　TFAs　in　practical　samples,　which　possesses　short　time-consuming,　low　disturbance　and　high　detection　sensitivity.　©　2016　Elsevier　B.V.