Highly efficient enzymatic preparation of isomalto-oligosaccharides from starch using an enzyme cocktail - Details

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Abstract：

Background　Current　commercial　production　of　isomalto-oligosaccharides　(IMOs)　commonly　involves　a　lengthy　multistage　process　with　low　yields.　Results　To　improve　the　process　efficiency　for　production　of　IMOs,　we　developed　a　simple　and　efficient　method　by　using　enzyme　cocktails　composed　of　the　recombinant　Bacillus　naganoensis　pullulanase　produced　by　Bacillus　licheniformis,　α-amylase　from　Bacillus　amyloliquefaciens,　barley　bran　β-amylase,　and　α-transglucosidase　from　Aspergillus　niger　to　perform　simultaneous　saccharification　and　transglycosylation　to　process　the　liquefied　starch.　After　13 h　of　reacting　time,　49.09%　IMOs　(calculated　from　the　total　amount　of　isomaltose,　isomaltotriose,　and　panose)　were　produced.　Conclusions　Our　method　of　using　an　enzyme　cocktail　for　the　efficient　production　of　IMOs　offers　an　attractive　alternative　to　the　process　presently　in　use.　©　2016

Keyword：

Amylases Aspergillus Bacteriology Oligosaccharides Saccharification Starch

Community：

[ 1 ] [Niu, Dandan]Fujian Provincial Key Laboratory of Marine Enzyme Engineering, College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350116, China
[ 2 ] [Niu, Dandan]The College of Biotechnology, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 3 ] [Niu, Dandan]The Key Lab for Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 4 ] [Qiao, Jian]The College of Biotechnology, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 5 ] [Qiao, Jian]The Key Lab for Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 6 ] [Li, Pujun]The College of Biotechnology, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 7 ] [Li, Pujun]The Key Lab for Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 8 ] [Tian, Kangming]Department of Biological Chemical Engineering, College of Chemical Engineering and Material Sciences, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 9 ] [Tian, Kangming]The Key Lab for Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 10 ] [Liu, Xiaoguang]Department of Biological Chemical Engineering, College of Chemical Engineering and Material Sciences, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 11 ] [Liu, Xiaoguang]The Key Lab for Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 12 ] [Singh, Suren]Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa
[ 13 ] [Lu, Fuping]The College of Biotechnology, Tianjin University of Science and Technology, Tianjin; 300457, China
[ 14 ] [Lu, Fuping]The Key Lab for Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin; 300457, China

Reprint 's Address：

[niu, dandan]the college of biotechnology, tianjin university of science and technology, tianjin; 300457, china;;[niu, dandan]fujian provincial key laboratory of marine enzyme engineering, college of biological science and engineering, fuzhou university, fuzhou; 350116, china;;[niu, dandan]the key lab for fermentation microbiology, ministry of education, tianjin university of science and technology, tianjin; 300457, china

Email：

ddniu0529@fzu.edu.cn

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Related Keywords：

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Source ：

Electronic Journal of Biotechnology

Year： 2017

Volume： 26

Page： 46-51

1 . 8 8 1

JCR@2017

2 . 3 0 0

JCR@2023

ESI HC Threshold：231

JCR Journal Grade：3

CAS Journal Grade：4

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 35

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

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