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author:

Niu, Dandan (Niu, Dandan.) [1] | Qiao, Jian (Qiao, Jian.) [2] | Li, Pujun (Li, Pujun.) [3] | Tian, Kangming (Tian, Kangming.) [4] | Liu, Xiaoguang (Liu, Xiaoguang.) [5] | Singh, Suren (Singh, Suren.) [6] | Lu, Fuping (Lu, Fuping.) [7]

Indexed by:

EI Scopus SCIE

Abstract:

Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. Results: To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant Bacillus naganoensis pullulanase produced by Bacillus licheniformis, alpha-amylase from Bacillus amyloliquefaciens, barley bran beta-amylase, and alpha-transglucosidase from Aspergillus niger to perform simultaneous saccharification and transglycosylation to process the liquefied starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose, isomaltotriose, and panose) were produced. Conclusions: Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive alternative to the process presently in use. (C) 2017 Pontificia Universidad Cat\u00f3lica de Valpara\u00edso. Production and hosting by Elsevier B.V. All rights reserved.

Keyword:

alpha-Amylase alpha-Transglucosidase Aspergillus niger Bacillus amyloliquefaciens Bacillus licheniformis Bacillus naganoensis pullulanase Barley bran beta-amylase Iisomalto-oligosaccharides production Saccharification Ttransglycosylation

Community:

  • [ 1 ] [Niu, Dandan]Fuzhou Univ, Coll Biol Sci & Engn, Fujian Prov Key Lab Marine Enzyme Engn, Fuzhou 350116, Peoples R China
  • [ 2 ] [Niu, Dandan]Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
  • [ 3 ] [Qiao, Jian]Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
  • [ 4 ] [Li, Pujun]Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
  • [ 5 ] [Lu, Fuping]Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
  • [ 6 ] [Tian, Kangming]Tianjin Univ Sci & Technol, Coll Chem Engn & Mat Sci, Dept Biol Chem Engn, Tianjin 300457, Peoples R China
  • [ 7 ] [Liu, Xiaoguang]Tianjin Univ Sci & Technol, Coll Chem Engn & Mat Sci, Dept Biol Chem Engn, Tianjin 300457, Peoples R China
  • [ 8 ] [Niu, Dandan]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China
  • [ 9 ] [Qiao, Jian]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China
  • [ 10 ] [Li, Pujun]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China
  • [ 11 ] [Tian, Kangming]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China
  • [ 12 ] [Liu, Xiaoguang]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China
  • [ 13 ] [Lu, Fuping]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China
  • [ 14 ] [Singh, Suren]Durban Univ Technol, Dept Biotechnol & Food Technol, Durban, South Africa

Reprint 's Address:

  • 牛丹丹

    [Niu, Dandan]Fuzhou Univ, Coll Biol Sci & Engn, Fujian Prov Key Lab Marine Enzyme Engn, Fuzhou 350116, Peoples R China;;[Niu, Dandan]Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China;;[Tian, Kangming]Tianjin Univ Sci & Technol, Coll Chem Engn & Mat Sci, Dept Biol Chem Engn, Tianjin 300457, Peoples R China;;[Niu, Dandan]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China;;[Tian, Kangming]Tianjin Univ Sci & Technol, Minist Educ, Key Lab Fermentat Microbiol, Tianjin 300457, Peoples R China

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Source :

ELECTRONIC JOURNAL OF BIOTECHNOLOGY

ISSN: 0717-3458

Year: 2017

Volume: 26

Page: 46-51

1 . 8 8 1

JCR@2017

2 . 3 0 0

JCR@2023

ESI Discipline: BIOLOGY & BIOCHEMISTRY;

ESI HC Threshold:231

JCR Journal Grade:3

CAS Journal Grade:4

Cited Count:

WoS CC Cited Count: 25

SCOPUS Cited Count: 38

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

Online/Total:135/10018980
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