Indexed by:
Abstract:
Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. Results: To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant Bacillus naganoensis pullulanase produced by Bacillus licheniformis, alpha-amylase from Bacillus amyloliquefaciens, barley bran beta-amylase, and alpha-transglucosidase from Aspergillus niger to perform simultaneous saccharification and transglycosylation to process the liquefied starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose, isomaltotriose, and panose) were produced. Conclusions: Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive alternative to the process presently in use. (C) 2017 Pontificia Universidad Cat\u00f3lica de Valpara\u00edso. Production and hosting by Elsevier B.V. All rights reserved.
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Source :
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
ISSN: 0717-3458
Year: 2017
Volume: 26
Page: 46-51
1 . 8 8 1
JCR@2017
2 . 3 0 0
JCR@2023
ESI Discipline: BIOLOGY & BIOCHEMISTRY;
ESI HC Threshold:231
JCR Journal Grade:3
CAS Journal Grade:4
Cited Count:
WoS CC Cited Count: 25
SCOPUS Cited Count: 38
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 2
Affiliated Colleges: