The　aim　of　this　study　was　to　optimize　the　technology　of　preparation　of　antioxidant　peptides　from　Chinese　leek　seeds　protein　hydrolysates　using　response　surface　methodology　(RSM).　The　proteins　extracted　from　Chinese　leek　seeds　were　hydrolyzed　by　acid-protease　and　antioxidant　activity　of　the　hydrolysates　was　evaluated　by　DPPH　radical　scavenging　method.　Effects　of　pH,　substrate　concentration,　enzyme　dosage　and　temperature　on　DPPH　scavenging　activity　of　hydrolysates　were　evaluated.　Based　on　the　results　of　the　single　factor　experiments,　a　procedure　optimized　using　the　response　surface　analysis　with　three　factors　(pH,　enzyme　dosage　and　hydrolysis　time)　was　conducted　in　order　to　obtain　the　optimal　enzymolysis　condition.　The　quadratic　term　model　of　the　enzymatic　preparation　technology　was　established　and　the　reliability　was　verified.　The　interaction　effects　of　pH,　enzyme　dosage　and　time　were　investigated,　and　the　optimal　hydrolysis　condition　was　gained　and　checked　out　experimentally.　The　results　indicated　that　the　effect　order　of　the　three　factors　on　antioxidant　activity　of　protein　hydrolysates　was　pH　＞　enzyme　dosage　＞　hydrolysis　time,　and　the　optimal　enzymolysis　conditions　were　time　6.5　h,　pH　3.00　and　enzyme　dosage　1345.7　U.　Under　the　optimal　enzymolysis　conditions,　DPPH　radical　scavenging　rate　of　the　hydrolysates　was　80.12%,　which　indicated　that　the　protein　hydrolysates　possessed　strong　antioxidant　activity.　©　2016,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.