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Abstract:
A pectinase-producing strain-Aspergillus niger YQ-13 was mutagenized with UV-LiCl and the mutant YQY-36 was obtained. The pectinase activity of the mutant YQY-36 was increased to 67.6 U/mL, 2.44 times higher than that of the original strain YQ-13. Plackett-Burman, steepest ascent and central composite designs were employed to optimize the fermentation media of YQY-36 in this study. The results showed that the optimum medium contained (g/L): orange peel 42.5, glucose 5.0, peptone 28.5, (NH4)2SO4 15.0, Na2HPO4·12H2O 1.7, KH2PO4 1.4, ZnSO4·7H2O 0.5. As a result, the maximum pectinase activity of strain YQY-36 was 92.1 U/mL and increased 36.2% after optimization. © 2016, Chinese Institute of Food Science and Technology. All right reserved.
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Journal of Chinese Institute of Food Science and Technology
ISSN: 1009-7848
CN: 11-4528/TS
Year: 2016
Issue: 1
Volume: 16
Page: 99-107
Cited Count:
SCOPUS Cited Count: 1
ESI Highly Cited Papers on the List: 0 Unfold All
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30 Days PV: 1
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