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Abstract：
A pectinase-producing strain G1 was isolated from rotten fruit by the methods of transparent circle observation and pectinase activity determination. Strain G1 was identified as Aspergillus niger based on its morphological observation and analysis of 18S rDNA sequence. Through single-factor experiments, the optimum medium contains (g/L): orange peel 32, bran 10, (NH4)2SO4 22, Na2HPO4·12H2O 8.4, KH2PO4 1.4, MgSO4·7H2O 1.0, Ca2Cl 0.1, FeSO4·7H2O 0.1, ZnSO4·7H2O 0.1, initial pH 6.5. The conditions for optimum pectinase-producing were the combination of the inoculums size 9%, medium volume 50 mL/250 mL, temperature 32°C , and shaking at 180 r/min for 7 days. Under these conditions, the pectinase activity which reached up to 270.4 U/mL was improved by 8.7 times compared with original pectinase activity.
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Journal of Chinese Institute of Food Science and Technology
ISSN： 1009-7848
CN： 11-4528/TS
Year： 2014
Issue： 2
Volume： 14
Page： 138-145
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ESI Highly Cited Papers on the List： 0 Unfold All
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30 Days PV： 2
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