For　the　situation　of　multiple　priority　requests,　the　serve　sequence　is　one　of　several　key　factors　that　have　a　significant　impact　on　the　efficiency　of　Transit　Signal　Priority　(TSP)　strategies.　The　serve　sequence　relates　to　the　TSP　parameter　settings　and　the　priority　phase　settings.　Therefore,　the　TSP　optimization　for　multiple　priority　requests　should　consider　the　different　combinations　of　priority　phase　settings　in　TSP　strategies.　In　order　to　achieve　that　objective,　this　paper　presents　a　cycle　optimization　method　for　multiple　TSP　requests　based　on　VISSIM-based　GA.　The　optimization　method　uses　the　length　of　signal　cycle　as　its　optimization　time　interval.　This　can　not　only　consider　the　vehicle　arrivals　before　the　priority　requests,　but　can　also　consider　the　vehicle　arrivals　after　the　priority　requests.　The　optimization　method　uses　VISSIM-based　GA　to　optimize　the　TSP　timing　on　a　single　signal　cycle　with　different　combinations　of　priority　phase　settings.　The　optimization　method　uses　the　VISSIMCOM　functions　“Save　Snapshot”　and　“Load　Snapshot”　to　ensure　the　VISSIM-based　GA　optimization　of　each　signal　cycle　starts　with　the　same　initial　traffic　state.　The　experiments　result　shows　that　the　cycle　optimization　with　different　priority　phase　settings　can　significantly　improve　the　precision　of　VISSIM-based　GA　optimization　when　the　TSP　strategy　is　effective　for　the　simulation　program.　The　impact　of　previous　cycle　optimization　will　be　offset　by　the　next　cycle　optimization　which　can　also　handle　traffic　fluctuations.　The　optimization　results　are　generated　by　the　combinations　of　cycle　optimization,　VISSIM-based　GA,　and　priority　phase　settings.　©　2016,　UK　Simulation　Society.　All　Rights　Reserved.