Isolation and characterization of a lutein-protein complex from Chlorella vulgaris - Details

author：

Cai, Xixi (Cai, Xixi.) ^[1] | Huang, Qimin (Huang, Qimin.) ^[2] | Yang, Qian (Yang, Qian.) ^[3] | Lin, Xiangzhi (Lin, Xiangzhi.) ^[4] | Wang, Shaoyun (Wang, Shaoyun.) ^[5]

Indexed by：

EI Scopus PKU CSCD

Abstract：

Lutein,　a　kind　of　indispensable　pigment　of　light　-harvesting　-complexs,　is　widely　distributed　in　higher　plants　and　algaes.　A　lutein-protein　complex　(LPC)　was　purified　from　Chlorella　vulgaris　by　the　procedures　including　solubilization　of　thylakoid　proteins,　ammonium　sulfate　precipitation　and　anion　exchange　chromatography.　The　molecular　weight　of　purified　LPC　was　29.0　kDa　by　SDS-PAGE.　Wavelength　scanning　confirmed　that　lutein　is　the　main　pigment　of　the　complex.　LPC　possessed　a　lutein:Chi:protein　ratio　of　about　3:1:1　according　to　HPLC　and　spectrophotometry　analysis.　The　complex　showed　strong　ABTS　radical　scavenging　activity　with　a　half　elimination　concentration　of　80　μg/mL.　©　2015,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.

Keyword：

Antioxidants Nitrogen compounds Proteins Purification Sulfur compounds

Community：

[ 1 ] [Cai, Xixi]College of Chemistry, Fuzhou University, Fuzhou; 350108, China
[ 2 ] [Cai, Xixi]College of Biological Science and Technology, Fuzhou University, Fuzhou; 350108, China
[ 3 ] [Huang, Qimin]College of Biological Science and Technology, Fuzhou University, Fuzhou; 350108, China
[ 4 ] [Yang, Qian]College of Biological Science and Technology, Fuzhou University, Fuzhou; 350108, China
[ 5 ] [Lin, Xiangzhi]Third Institute of Oceanography, State Oceanic Administration, Xiamen; Fujian; 361005, China
[ 6 ] [Wang, Shaoyun]College of Biological Science and Technology, Fuzhou University, Fuzhou; 350108, China